Asian Journal of Research in Chemistry

N. Senthilkumar ¹, B. Jayalakshmi ¹, Joicy Jose ¹, K. Sumathi ¹

Affiliations
1 Department of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, B. Komarapalayam, Namakkal, Tamil Nadu – 638 183, IN

Abstract

The chalcone was synthesized from cyclohexanone and an aromatic aldehyde by aldol condensation reaction, followed by cyclisation with thiourea results in hexahydro quinazoline. The third position is methylated by using methyl iodide, finally the mannich reaction was carried out with five different amines at second position of the hexahydro quinazoline to get five different S-Mannich bases. All the 5 synthesized new compounds were studied for their physiochemical characters. And their structures were confirmed by spectral studies (IR, NMR). These new derivatives were evaluated for analgesic and anti-inflammatory activity.

Keywords

S-Mannich Bases, Quinazoline, Chalcone.

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D. Kamalakannan ¹, R. Vijay Amirtharaj ¹, N. Senthilkumar ¹

Affiliations
1 J.K.K. Munirajah Medical Research Foundation, College of Pharmacy, Komarapalayam-638183, Namakkal Dt, Tamilnadu, IN

Abstract

A simple, precise, accurate, rapid and reproducible RP-HPLC procedure was developed for simultaneous estimation of Telmisartan and Pioglitazone in pharmaceutical dosage form at a single wavelength. The mobile phase used was a combination of acetonitrile:0.5% triethylamine (pH 4.5 with orthophosphoric acid) (65:35). The detection of the combined dosage form was carried out at 210 nm and flow rate set 1.3 ml/mins. Linearity obtained in the concentration range of 10 to 50 μg/ml for Telmisartan and 7.5 to 37.5 μg/ml for Pioglitazone with correlation coefficient of 0.9997 and 0.9995 respectively. The result of analysis were validated statistically recovery studies confirmed by the accuracy of the proposed method.

Keywords

Simultaneous Estimation by RP-HPLC, Telmisartan, Pioglitazone.

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S. Prabhu ¹, R. Vijay Amirtharaj ¹, N. Senthilkumar ¹

Affiliations
1 J.K.K. Munirajah Medical Research Foundation, College of Pharmacy, Komarapalayam-638183, Namakkal Dt, Tamilnadu, IN

Abstract

A simple, precise, accurate, rapid and reproducible RP-HPLC procedure was developed for simultaneous determination of Cefixime and Ofloxacin in tablet dosage form at a single wavelength. The determination was carried out by using Phenomenox, Gemini C18, Column (50×4.6 mm) with mobile phase used as a combination of acetonitrile:0.05 M Tetra butyl ammonium Hydrogen Sulphate (pH 6.0 with orthophosphoric acid) (35:65). The detection of the combined dosage form was carried out at 290 nm and flow rate set 2.0 ml/min. Linearity obtained in the concentration range of 160 and 240 Cefixime and Ofloxacin with correlation coefficient of 0.9999 and 0.9999 respectively. The result of the analysis were validated statistically and recovery studies confirmed by the accuracy of the proposed method.

Keywords

Cefixime, Ofloxacin and RP-HPLC.

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M. Sathiyaraj ¹, R. Vijay Amirtharaj ¹, N. Senthilkumar ¹

Affiliations
1 Department of Pharmaceutical Analysis, J.K.K.Munirajah Medical Research Foundation College of Pharmacy, The Dr. M.G.R. Medical University, Komarapalayam-638183, Namakkal Dt, Tamilnadu, IN

Abstract

A sensitive liquid chromatography-electro spray ionization mass spectrometry (LC-ESI-MS) method is developed and validated for rapid determination of Esomeprazole in human plasma. Rabeprazole was used as the internal standard (I.S). Human plasma (80 μl) was first alkalified with 200 ml of sodium bi carbonate (100 mM) and then extracted with 2 ml of Ethyl acetate by vibromax shaker for 15 min. The mixture was centrifuged at 4000 rpm for 15 mins. The supernatant was evaporated to dryness and the residue was reconstituted with water: 0.02% Diethylamine in methanol (3:7 v/v). Samples were separated by using a Gemini C₁₈ reversed phased column (50 mm×4.6 mm I.D, 5μ). Mobile phase consisted of Acetonitrile:water (pH:7.0 with Ammonia) [8:2 v/v]. Esomeprazole and internal standard were measured by electrospray ion source in positive selective ion monitoring mode. The good linearity ranged from 5 ng/ml to 1000 ng/ml and the lowest limit of quantification was 5 ng/ml. The extraction efficiency was approximately 73.4%. The quality control samples were stable when kept at room temperature for 6 hours, -70°C for 29 days and after three freeze- thaw cycles.

Keywords

Esomeprazole, Rabeprazole, LCMS/MS.

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