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Authors
Affiliations
1 Department of Pharmaceutical Analysis, JKK Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam-638183, Namakkal (DT), Tamilnadu, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 4 (2010), Pagination: 1062-1064
Abstract
The present paper deals with the development of RP-HPLC method for the determination of Nitazoxanide and Ofloxacin in bulk and in formulation using UV detector. Selected mobile phase was a combination of 0.005 M tetra butyl ammonium hydrogen sulphate and acetonitrile in the ratio of 45:55 and the wavelength selected was 240 nm. The flow rate 1.0 ml/min, and the injection volume was 20 μl. The sepration was performed at ambient temperature. Retention time of Nitazoxanide and Ofloxacin was found to be 6.051and 2.106. Linearity of the method was found to be 400 to 600 mcg/ml and 160 to 240 mcg/ml for Nitazoxanide and Ofloxacin respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.08-99.81% and 100.18-100.65% for Nitazoxanide and Ofloxacin respectively. This method was validated according to ICH guidelines.
Keywords
Nitazoxanide, Ofloxacin and RP-HPLC.
