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Authors
Affiliations
1 Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune-411038, Maharashtra, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 3 (2009), Pagination: 266-269
Abstract
A simple, sensitive, selective, precise and reversed-phase stability-indicating HPLC method was developed and validated for Bumetanide (BUM) in bulk drug and in formulation. The method has shown adequate separation for BUM from their associated impurities and their degradation products. Separation was achieved on a Waters Spherisorb ODS2 C18, 250 mm×4.6 mm i.d., 5 μm column using a mobile phase consisting of methanol: water (70:30, v/v) at a flow rate of 1 ml/min and UV detection at 335 nm and the retention time (TR) was found to be 4.743±0.05 min. The method was also applied for the determination of BUM in the presence of their degradation products formed under variety of stress conditions. Proposed method was validated for precision, accuracy, linearity range, recovery and robustness.
Keywords
Reverse Phase HPLC, Bumetanide, Forced Degradation.
