Asian Journal of Research in Chemistry

P. Asha ¹, I. Ponnilavarasan ¹, Ch. Sunil Narendara Kumar ¹, J. Dharuman ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, K.M.C.H College of Pharmacy, Kalapatti Road, Coimbatore-641048, Tamilnadu, IN

Abstract

A simple, precise, rapid, selective and economical high performance thin layer chromatographic (HPTLC) method has been established for simultaneous analysis of Esomeprazole and Domperidone in capsule dosage forms. The chromatographic separation was performed on precoated silica gel 60 GF254 plates with Acetonitrile: Chloroform: Ammonia (12:7. 5:0. 5)(v/v/v)as a mobile phase. The drugs on the plate were scanned at 222 nm. The drugs were satisfactorily resolved with Rf 0. 31 for domperidone and 0. 48 for esomeprazole. The method was validated for linearity, accuracy, precision, and specificity. The calibration plot was linear between 200-1000 ng/spot for esomeprazole and domperidone. The limit of detection and quantification for esomeprazole was 85. 07 and 255. 21ng/spot and domperidone was 79. 07 and 237. 23 ng/spot. The percent recovery study was done by standard addition method found in the range of 98. 7% and 99. 1% respectively. The proposed HPTLC method is economic, sensitive, and less time consuming than other chromatographic procedures.

Keywords

Denisitometry, Quantification, Esomeprazole, Domperidone.

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I. Ponnilavarasan ¹, A. Rajasekaran ¹, K. K. Sivakumar ¹, C. Sundaramoorthi ¹, Swastika Ganguly ², R. Sivasakthi ³

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore-641048, IN
2 Birla Institute of Technology, Mesra, Ranchi, IN
3 Cherrann’s College of Pharmacy, Coimbatore, Tamilnadu, IN

Abstract

A number of organic compounds obtained by chemical synthesis as model compounds have useful antimicrobial activities. Quinazoline ring is an aromatic benzopyrimidine system;many of its derivatives possess interesting biological activities, such as analgesic, anti-inflammatory, anti-microbial, and anti-tumor. In our study, the biological activity of synthesized quinazoline semicarbazone derivatives were characterized by antimicrobial screening against several gram-positive, gram negative bacteria, and fungus. The purity of the synthesized compounds was characterized by physicochemical properties, such as solubility, melting point, and thin layer chromatography (TLC). Elemental analysis for carbon, nitrogen, hydrogen, and oxygen was performed according to standard procedures. The presence of functional groups was analyzed using FT-IR spectra. Molecular structural information for the compounds was analyzed by 1H-NMR spectroscopy. The wavelengths of maximum absorbance for all the synthesized compounds were measured by UV-Visible spectroscopy. Thereby the chemical structures of the synthesized quinazoline derivatives were confirmed. Antimicrobial screening for all the compounds exhibits characteristic microbial inhibition. A detailed study is in progress to modify the synthetic route, structural activity, and toxicological barriers for the enhanced pharmacological efficiency of synthetic antibiotics.

Keywords

Antimicrobial Activity, Quinazoline Semicarbazone, Biological, Synthetic Antibiotics.

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