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Vidya Sagar, G.
- Simultaneous Estimation of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in Pharmaceutical Dosage Forms by RP-HPLC Method
Authors
1 CRO, Veeda, Ahmedabad, IN
2 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, IN
3 Department of Pharmaceutical Sciences, Saurastra University, Rajkot, Gujarat, IN
4 CVM Institute for Degree Course in Pharmacy, New Vallabh Vidhyanagar, IN
5 Ipcowala Indukaka College of Pharmacy, Vallabh Vidhyanagar, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 1 (2010), Pagination: 83-86Abstract
A simple, specific, accurate and stability indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of and Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in tablet dosage forms. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27 μm size, 250 mm´4.6 mm i.d.) at ambient temperature, in isocratic mode, with mobile phase containing Methnol: Acetonitrile: Phosphate Buffer, pH 5.39 (20: 40: 40% v/v/v) adjusted to pH 5.39 using ortho phosphoric acid was used. The flow rate was 1.0 ml/min and effluents were monitored at 238 nm. The retention times of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride were 3.69 min, 8.18 min and 12.5 min respectively.The calibration curves were linear in the concentration range of 4-24 μg/ml for Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride. The proposed method was validated and successfully applied to the estimation of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in combined tablet dosage forms. Linearity was obtained in the concentration range of 4 to 24 μg/ml of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride with a correlation coefficient of 0.9963, 0.9992 and 0.9957 respectively. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27 μm size, 250 mm, 4.6 mm i.d.) at ambient temperature. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in routine analysis.
Keywords
Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride, RP-HPLC.- Simultaneous Estimation of Lansoprazole and Domperidone in Combined Dosage Form by RP-HPLC
Authors
1 Ramanbhai Patel College of Pharmacy, Changa, Gujarat, IN
2 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, Gujarat, IN
3 Veeda, Ahmedabad, Gujarat, IN
4 A.R. College of Pharmacy, Vallabh Vidhyanagar, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 2 (2009), Pagination: 210-212Abstract
A RP-HPLC method has developed for simultaneous estimation of lansoprazole and domperidone in combined capsule dosage form. Lansoprazole is a proton pump inhibitor and used in the treatment of duodenal and gastric ulcers and gastroesophageal reflux disease (GERD), a condition in which the acid in the stomach washes back up into the esophagus. Sometimes lansoprazole is used in combination with antibiotics to treat ulcers associated with infection caused by the H. pylori bacteria, gastro-oesophageal reflux disease (GERD), peptic ulcer and Zollinger-Ellison syndrome. Domperidone is indicated for treating symptoms associated with upper gastrointestinal motility disorders caused by chronic and sub acute gastritis. It is a gastrointestinal emptying (delayed) adjunct, a peristaltic stimulant and exhibit antiemetic properties.
The mobile phase used was a combination of Acetonitrile: Methanol (81:19). The detection of the combined dosage form was carried out at 280 nm and a flow rate employed was 1 ml/min. The retention time for lansoprazole and domperidone has found to be 2.8 and 1.57 min respectively. Linearity was obtained in the concentration range of 8-24 μg/ml of lansoprazole and 8-24 μg/ml of domperidone with a correlation coefficient of 0.9977 and 0.9992. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27μm size, 250 mm´4.6 mm i.d.) at ambient temperature. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of domperidone and lansoprazole in routine analysis.
Keywords
Simultaneous Estimation, RP-HPLC, Lansoprazole(LAN), Domperidone(DOM).- RP-HPLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Combined Dosage Forms
Authors
1 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, Gujarat, IN
2 A.R. College of Pharmacy, Vallabh, Vidhyanagar, Gujarat, IN
3 Department of Pharmaeutical Sciences, Saurastra University, Rajkot, Gujarat, IN