Asian Journal of Research in Chemistry

Mayur G. Raval ¹, Preeti Tiwari ¹, C. N. Patel ¹

Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug , Mehsana-384001, Gujarat, IN

Abstract

The Objective Of the current study was to develop a validated stability indicating high performance liquid chromatographic method for Pramipexole in solid dosage form. The method was validated by subjecting the drugs to forced decomposition under hydrolysis, Oxidation, photolysis, and thermal stress conditions prescribed in international Conference on Harmonization. The drug was successfully separated from major and minor degradation products on a reversed -phase Zorbex SB CN column by using Tri Ethyl Amine buffer (PH 7): Methanol (65:35%V/V) as the mobile phase with determination at 263 nm. The flow rate was 1 ml/min. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 2-24 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.60-101.84%. The utility of the procedure was verified by its application to marketed formulations that were subjected to accelerated stability studies.

Keywords

Pramipexole, Stability Assay, High Performance Liquid Chromatography, Validation, Mirapex.

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Mayur G. Raval ¹, Preeti Tiwari ¹, C. N. Patel ¹

Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

The Objective Of the current study was to develop and validated UV spectrophotometric method for Pramipexole in solid dosage form. The method was validated by subjecting the drug to UV radiation. The drug was successfully quantified at 263 nm. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 3-15 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.00-101.46%. The utility of the procedure was verified by its application to marketed formulations. The specificity of the method was also checked with reference to placebo. The method was found to be specific also, hence other validation parameter were in the limit also.

Keywords

Pramipexole, Assay, UV Spectrophotometric, Validation, Mirapex.

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Yatri R. Shah ¹, Dhrubo Jyoti Sen ¹, C. N. Patel ¹

Affiliations
1 Department of Pharmaceutical and Medicinal Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

CYP450 exists in prokaryotic and eukaryotic (plants, insects, fish and mammal, as well as microorganism).Different P450 enzymes can be found in almost any tissue:liver, kidney, lungs and even brain. It plays an important role in drugs metabolism and xenobiotics. Cytochrome P450 proteins in humans are drug metabolizing enzymes and enzymes that are used to make cholesterol, steroids and other important lipids such as prostacyclins and thromboxane-A2.Firstly CYP450 is discovered by R.T. Williams-in vivo, 1947. Brodie-in vitro, from late 40s till the 60s. Cytochrome P450 enzymes (hemoproteins) play an important role in the intra-cellular metabolism CYP enzymes have been identified from all lineages of life, including mammals, birds, fish, insects, worms, sea squirts, sea urchins, plants, fungi, slime molds, bacteria and archaea. More than 8100 distinct CYP sequences are known. CYP450 includes hydroxylation and various xenobiotic reactions. Clinical aspects of CYP450 include genetic polymorphism and drug drug interaction. Roche Amplichip test in very useful nowadays because many harmful reactions resulting from inappropriate dosing and treatment may be significantly reduced as clinicians can adjust the patient’s regimen accordingly. The AmpliChip Cytochrome P450 Genotyping System may help the doctor determine if a patient is at risk of adverse drug reactions or sub-optimal drug response. Thus it is very up growing technique to prevent adverse drug reactions.

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Parimal M. Prajapati ¹, Yatri Shah ¹, D. J. Sen ¹, C. N. Patel ¹

Affiliations
1 Department of Pharmaceutical and Medicinal Chemistry, Shri Sarvajanik Pharmacy College, Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

Combinatorial chemistry is a technology for creating a multitude of different compounds by reacting different combinations of interchangeable chemical "building blocks." The compounds are then screened for their ability to carry out a specified function, most commonly to act as drugs to treat a disease. Combinatorial chemistry allows the rapid synthesis and testing of many related compounds, greatly speeding the pace of drug discovery. Combinatorial chemistry is a method for reacting a small number of chemicals to produce simultaneously a very large number of compounds, called libraries, which are screened to identify useful products such as drug candidates. combinatorial chemistry can be explained simply, its application can take a variety of forms, each requiring a complex interplay of classical organic synthesis techniques, rational drug design strategies, robotics, and scientific information management. Combinatorial technologies offer significant advances over traditional scientific research methodologies. In particular, their high-speed approach promises faster results at considerably lower costs than conventional techniques. combinatorial chemistry libraries requires an application that understands the science behind combinatorial chemistry while managing the chemical and biological data generated by combinatorial chemistry programs.

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Vijay K. Patel ¹, Chirag K. Patel ¹, Harsha U. Patel ¹, C. N. Patel ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

Antioxidants neutralize free radicals. Free radicals are molecules in our body that are missing an electron. They steal electrons from healthy cells destroying them in the process called oxidation. Your body is constantly trying to oxidize, but antioxidants keep you fresh and healthy. It prevents the formation of free radicals, such as transferrin, SOD, carotenoids. It neutralise those that are formed, thus inhibiting chain-breaking processes, such as, the vitamins A, E and C. Also it repair the damage caused by free radicals, such as the DNA repair enzymes, e.g. transferase. Natural antioxidants are synthesised by plants and are present in the foods we eat, as opposed to those synthetic antioxidants that are either added to food to extend its shelf-life (e.g. BHT), or prepared by extraction from plant sources to be taken as supplements in concentrated form.

Keywords

Free Radicals, SOD, Shelf Life, BHT.

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Vishal S. Thakar ¹, Chirag K. Patel ¹, H. U. Patel ¹, C. N. Patel ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

Approximately 50 NSAID preparations are listed in Monthly Index of Medical Specialties and, as a class, these are among the most commonly prescribed drugs. NSAIDs are sometimes known as the aspirin-like drugs because they have an activity profile that is broadly similar to that of aspirin that is, they all possess analgesic, anti-inflammatory and antipyretic properties to some degree, and produce characteristic side effects, including gastric intolerance and depression of blood clotting through inhibitory action on platelet function. Two closely related forms of the cyclooxygenase have been identified which are now known as COX-1 and COX-2. Both isoenzymes transform arachidonic acid to prostaglandins, but differ in their distribution and their physiological roles. Meanwhile, the responsible genes and their regulation have been clarified. COX-1, the pre-dominantly constitutive form of the enzyme, is expressed throughout the body and performs a number of homeostatic functions such as maintaining normal gastric mucosa and influencing renal blood flow. COX-1 and COX-2 at standard anti-inflammatory doses. Simmons also recently co-discovered COX-3 in 2002 and analyzed this new isozyme's relation to acetaminophen (paracetamol), arguably the most widely used analgesic drug in the world. The clinical ramifications and knowledge of COX isozymes are therefore rapidly expanding and could perhaps offer significant hope for future treatments of pain, inflammation and fever.

Keywords

Liquid Chromatography, Mass Spectrometry Valsartan.

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Prashant S. Mewada ¹, Chirag K. Patel ¹, C. S. Rami ¹, H. U. Patel ¹, C. N. Patel ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

The increasing prevalence of obesity worldwide has prompted the world health organization (WHO) to classify it as a global epidemic. A round the global, more than a half a billion people are overweight and the chronic disease of obesity represents a major threat to health care system in developed and developing countries. Energy homeostasis is accomplished through a highly integrated and reductant neurohumoral system. Adrenergic and seretonergic agents enjoyed before is now disfavored due to abuce and lack of exact receptor subtype profile respectively.ß3-adrenergic receptor agonist acting, as thermogenic agents are new approach and its value will become apparent once data are available from relevant clinical evaluation some drug from this class are under clinical trials. Transgenic technology has provided new opportunities to modify the complex body weight regulation system and to asses the relative importance of the individual components. Certain peptides have been used successfully as antiobesity agents. They reduced gastrointestinal absorption and affect feeding behavior. Since obesity result from genetic predispotion, combined with the proactive environment situation, we discuss new potential targets for generation of drugs that may help people in gaining control over appetite as well as increase total energy expenditure and fat oxidation.

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R. C. Patel ¹, C. N. Patel ¹, B. B. Panigrahi ¹, V. H. Bhaskar ¹

Affiliations
1 Department of Pharmaceutical and Medicinal Chemistry, Shri Sarvajanik Pharmacy College, Hemchandracharya North Gujarat University, Near Arvind Baug, Mehsana-384001, Gujarat, IN

Abstract

Some New benzhydryl piperazine sulfonamide and carboxamide is synthesized from benzaldehyde and phenyl magnesium chloride (Grignard Reagent) under nitrogen atmosphere to give benzhydrol which on treating with thionyl chloride give corresponding benzhydryl chloride which is directly treated with piperazine and anhydrous potassium carbonate using DMF as solvent to give corresponding 1-benzhydryl piperazine. The nucleophilic substitution reaction of 1-benzhydryl piperazine with different substituted aromatic sulfonyl chloride and acid chloride in presence of triethylamine and dichloromethane to give corresponding benzhydryl piperazine sulfonamide and carboxamide. All the title compounds characterised on the basis of their IR, MASS, ¹H NMR spectroscopic data analysis.

Keywords

Benzhydrol, Acid Chloride, Sulfonyl Chloride, Antimicrobial Screening.

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R. Badmanaban ¹, C. N. Patel ¹, P. Devi ², R. Meera ², B. Kameswari ², B. Eswarapriya ³

Affiliations
1 Shri Sarvajanik Pharmacy College, Mehasana, North Gujarat, IN
2 K.M. College of Pharmacy, Uthangudi, Madurai, IN
3 St. Michael College of Engineering, Kalayarkovil, Tamilnadu, IN

Abstract

Formulated oil a Siddha formulation was developed using two plant drugs namely Pongamia pinnata seeds and Boerhaavia diffusa ischolar_mains. Preliminary physio chemical screening was performed in both the plants. The wound healing activity was studied with comparison of Sesame oil and Formulated oil by using standard nitro furazone ointment. The methods used were Excision wound model, Incision wound model and Dead space wound model. In excision and incision wound model the formulated oil treated group showed much greater increase in the tensile strength when compared to the control, which was almost similar to that of nitro furazone. In the dead space wound model both the sesame oil and formulated oil produced a significant increase in the wet granuloma tissue weight as well as in the dry weight .Tensile strength was also found to be increased (P<0.001) in both of the oil treated groups.

Keywords

Pongamia pinnata, Boerhaavia diffusa, Sesame Oil, Formulated Oil, Wound Healing Activity.

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