A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Ahmed, Ashraf M.
- RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine
Authors
1 Prof. of Inorganic and Analytical Chemistry, Al-Azhar University, Cairo, EG
2 Forensic Chemist, Medico-Legal Organization, Ministry of Justice, Cairo, EG
Source
Asian Journal of Research in Chemistry, Vol 4, No 1 (2011), Pagination: 24-27Abstract
A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of amitriptyline hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 % v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection at 254nm. The retention time for amitriptyline hydrochloride was 7.3 min. The calibration curve was linear in the range 0.5-3 μg/mL. The mean recovery for amitriptyline hydrochloride is 100.025 %. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.