Asian Journal of Pharmacy and Technology

J. Madhusudhanan ¹, M. Indumathi ¹, S. Ammu ¹

Affiliations
1 Department of Biotechnology, Shri Andal Alagar College of Engineering (SAACE), Mamandur-603 111, IN

Abstract

Several biological drugs such as proteins and nucleic acids require novel delivery technologies that will minimize side effects and lead to better patient compliance. Hence, various research using nanoparticle as a carrier has been done for the delivery of gene drug. In the present work, delivery of proteases and plasmid DNA using nanoparticle as a carrier on E.colis was studied. Proteases, a proteolytic enzyme which is capable of cleaving the peptides are made inactive by inhibitors present inside the cell. But proteases can be made resistive by coating with nanoparticles. The action of protease has been analysed on a specific enzyme i.e. alkaline phosphatase. Along with the protease delivery, plasmid delivery is also done to develop a carrier capable of delivering drug and gene complex. Similarly, carrier system for drug and plasmid was studied and developed for cancer cells which were capable of delivering drugs as well as short polynucleotide complex. It was found that the cancer cells lines SW480 and A549 showed cell death due to the action of drugs. This novel carrier showed effective delivery of drug and high efficacy in action. From this study it was confirmed that a protein or drug can be conjugated with nanoparticles along with gene carrier to import both functions of drugs and gene on a targeted cell using single carrier. Such system can be developed specific genetic disorders such as Osteogenesis imperfecta, cancer.

Keywords

Plasmid, Protease, Cancer Cell, Osteogenesis imperfecta.

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J. Madhusudhanan ¹, S. Sandhya ¹, S. Malathi ¹

Affiliations
1 Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111, IN

Abstract

Many drugs are in the treatment of cancer, doxorubicin (DOX), a common cancer chemotherapeutics, was conjugated to folate-modified polyethyleneimine-functionalized gold nanoparticles. The controlled release behaviour of DOX-loaded gold nanoparticles was analysed using Tris HCl and PBS. The TEM and FTIR characterization says that there isa strong association of DOX over folate modified gold nanoparticle. DOX-loaded gold nanoparticles had higher cytotoxicity for folate-receptor-positive cells (KB cells) compared to folate-receptor-negative cells (A549 cells) which show lower cells viability at higher concentration of folate modified gold nanoparticle. The viability of KB cells is 7% and A549 cells are 17% at high concentration AuNP-PEI-FOL-DOX. The DOX effects on cancer cell DNA were determined by DNA cleavage pattern.

Keywords

Nanoparticles, Polymer, UV-VIS Spectroscopy, TEM, Tryphan Blue Assay.

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J. Madhusudhanan ¹, P. Monika ¹, K. Monica ¹

Affiliations
1 Department of Biotechnology, Shri Andal Alagar College of Engineering (SAACE), Mamandur-603 111, IN

Abstract

Recently nanoparticles have been developed for the photo acoustic imaging, delivery of genes and laser induced photo thermal therapy. In this study, we have developed ssDNA conjugated nanoparticles as the carrier for simultaneous DNA and anti-cancer nucleoside delivery. The ssDNA nanoparticle complex presented higher capacity in carrying anti-cancer drug compounds than the original nanoparticles. The hydrodynamic size of the nanoparticles increased from 25 to 35 nm with an increase in the negative surface charge from -9.58 to 21.66 mV after ssDNA conjugation and drug loading. A positive association between environmental pH and drug release was observed in PBS and Tris/HCl, which implied their potential use in the controlled localized drug release in the lower GI tract. The Tryphan Blue, Ethidium Bromide assay revealed dose dependent cytotoxicity to oral and lung cancer cell line than free compounds. These results suggest the potential use of this new ssDNA nanoparticles complex as the environmental controlled anticancer nanocapsule, especially suitable for oral and lung cancer chemotherapy.

Keywords

Anticancer, Nanocapsule, Drug Carrier, Cytotoxicity.

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J. Madhusudhanan ¹, P. Mohanambal ¹, V. Sowmya ¹

Affiliations
1 Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111, IN

Abstract

Nanoparticles are found to be useful as carrier for gene delivery. In the present study, the most biocompatible-Gold nanoparticles were used for carrying serine protease. i.e. Trypsin. It is sensitive to the protease inhibitors present inside the cells and these proteases on binding with gold nanoparticle, loses its stability but retain its activity. The Gold Nanoparticle along with the protease was characterized by UV-Vis spectroscopy, TEM and FTIR. The interaction was confirmed between Trypsin and Gold Nanoparticles and its size was found between 20-28nm. Further, the activity of Trypsin bound to Nanoparticles and the ability of AuNP's as carrier were analyzed by invitro studies on E.coli HB101. Trypsin's activity on intracellular protein and specifically on ALPase enzyme showed a 25-29% decrease in protein content which directs that Trypsin bound to Nanoparticles has found to be active within the cell. These results suggest that, such proteolytic enzyme can be designed with nanoparticle to lyse the defective proteins produced by the cells due to genetic disorder.

Keywords

Gold Nanoparticles, Trypsin, Alkaline Phosphatase, Protein.

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