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Authors
Affiliations
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
Source
Asian Journal of Pharmacy and Technology, Vol 5, No 2 (2015), Pagination: 83-90
Abstract
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Pravastatin and Valsartan in synthetic mixture using first order derivative zero-crossing method. Pravastatin showed zero crossing point at 262.40 nm while Valsartan showed zero crossing point at 248.20 nm. The dA/dλ was measured at 248.20 nm for Pravastatin and 262.80 nm for Valsartan and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.999) in the range of 2-10μg/ml for Pravastatin at 248.20 nm. The linear correlation was obtained (r2>0.9998) in the range of 8-40μg/ml for Valsartan at 262.40 nm. The limit of determination was 0.054μg/ml and 0.024μg/ml for Pravastatin and Valsartan, respectively. The limit of quantification was 0.166μg/ml and 0.074μg/ml for Pravastatin and Valsartan, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Pravastatin and Valsartan in binary mixture.
Keywords
Pravastatin, Valsartan, First Derivative Method, Spectroscopic Method.
