Asian Journal of Pharmacy and Technology

S. Gnanavel ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.

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K. Kaviyarasi ¹, K. Kanimozhi ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN

Abstract

The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.

Keywords

Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.

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A. Gayathri ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Department of Botany and Microbiology, A. V. V. M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The present study was to isolate and identify antimicrobial activity producing actinomycetes strains from marine habitat, to find out antibacterial efficiency against the common human pathogens. Marine actinomycetes were isolated from salt pan region of Kodiakarai, Nagapattinam [District].Out of 20 isolated actinomycetes 10 were identified and selected for antibacterial activity. Out of 10 dominant actinomycete species Streptoverticillium album was highly dominant and showed the best level of antibacterial activity against three human pathogens, Staphylococcus aureus, Klebsiella pheumoniae and Escherichia coli. The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics and also confirmed by molecular characterization.

Keywords

Antibacterial Activity, Sterptoverticillium album, Marine Actinomycetes.

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M. Prabakaran ¹, V. Thennarasu ¹, A. Panneerselvam ²

Affiliations
1 Sri Gowri Biotech Research Academy, No.5 Nagai Road, Thanjavur, 613 001, Tamilnadu, IN
2 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi 613 503, Tamilnadu, IN

Abstract

The learn of Sphaeranthus amaranthoides Burn, were examined for their antioxidant, antimutagenic, antimicrobial and phytochemical activities. Preliminary evaluation of both crude and solvent fractions showed a broad spectrum of activity since the extracts inhibit the growth of both bacterial and fungal isolates. The inhibitory activity of plant extracts on the peroxidation of linolic acid were measured by fericthiocynate methodo. Ethonolic extract showed strong antioxidant activity and antimicrobial activity against to the bacteria, (K.pneumoniae, S. typhi, E.coli, P.aeroginosa, S.aureus) and fungus (A.nidulans, A.terreus, A.sulphureus, Candida albicans and Fusarium sp.) cultures. Due to the phytochemical diversity in the antioxidant phytocompounds, the above results were expected. It almost rich in carotenes, Neoxanthin, chlorophyll a and chlorophyll b, lutein, violexanthin, pheophytin, which may have been responsible for the antioxidant, antimicrobial and for phytochemical activity. For antimutagenic activity using sodium azide to induce the chemical compound of Sphaeranthus amaranthoides Burn, to prevent the degenerative cells and results further support the view that some Indian medicinal plants are source for potential antioxidants.

Keywords

Medicinal Plants, Ferric Thiocyanate, Sodium Azide, Solvents, Human Pathogens, Thin Layer Chromatography.

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