Asian Journal of Pharmaceutical Research

Firoj A. Tamboli ¹, Harinath N. More ¹

Affiliations
1 Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, IN

Abstract

The objective of this study was to investigate the ant proliferative activity of the successive solvent extract of leaves of plant of Barleria gibsoni Dalz. against MDA MB 4355 (Human breast cancer) and Hep G2 (Liver cancer cell line). 5 fluorouracil was given as reference drug. The leaves of plant were dried in shade and they were powdered and extracted with different solvents extract like petroleum ether, chloroform, acetone, ethyl acetate, ethanol. The preliminary phytochemical tests were done. Pet ether, chloroform extract of leaves showed that significant ant proliferative effects were obtained against MDA MB 4355 (Human breast cancer) by SRB assay method and ethanol extract of leaves showed moderate activity against Hep G2 (Liver cancer cell line) by MTT assay method due to presence of phytoconstituents present in the plant.

Keywords

Barleria gibsoni Dalz. Ant Proliferative Activity, 5-Fluorouracil, MDA MB 4355 (Human Breast Cancer) and Hep G2 (Liver Cancer Cell Line).

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Suresh G. Killedar ¹, Harinath N. More ¹

Affiliations
1 Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur (M.S.), IN

Abstract

The plant Memecylon umbellatum Burm is small tree or shrub with beautiful purple inflorescences. Various other parts of the plant were studied for different medicinal properties but no scientific data on inflorescence except the description about its beauty and photographs. Hence in the present work efforts have been made to investigate antimicrobial properties and phytochemical screening of the inflorescence.

Six different extracts with non polar and polar solvents were screened for antimicrobial activity by cylinder cup plate method using standard cultures (ATCC), MIC by serial broth dilution and phytochemical screening was made with different chemical reagents. Ethyl acetate and methanol extracts have shown better activities compare to other extracts and were found significant compare to control and standard at tested concentrations. MIC for ethyl acetate and methanol extract was found at 0.5mg for most of the test organisms compare to 3 and 6mg for other extracts. All other extracts have shown very weak activity as compare to standard even at higher concentrations. Most of the non polar extracts showed almost negligible anti fungal and very weak antibacterial activities. Different extracts showed presence of tannins, glycosides, triterpens and steroids as important constituents.

Keywords

Antimicrobial, Phytochemical Screening, Memecylon umbellatum, Cylinder Cup Plate Method.

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