Asian Journal of Pharmaceutical Research

P. Madhanraj ¹, K. Ramanathan ², N. Arun ³, N. Nadimuthu ⁴, A. Panneerselvam ⁵

Affiliations
1 Department of Microbiology, Thanthai Hans Roever College, Perambalur-621212, IN
2 Department of Bioinformatics, Thanthai Hans Roever College, Perambalur-621212, IN
3 Department of Biochemistry, Thanthai Hans Roever College, Perambalur-621212, IN
4 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
5 Department of Botany and Microbiology, A.V.V.M Sri Puspam College, Poondi, Thanjavur–613503, Tamil Nadu, IN

Abstract

We have been investigating that the atomic study of Fusarium incarnatum with the utilization of Bioinformatics tools. We have been involved in the insilico study on the plant pathogen the Fusarium incarnatum. The involvement of Nucleotides and atoms in this species can be identified with the help of Bioinformatics tools. We can also involved in the analysis of amino acids which are present in this species and calculated the start and stop codons in different frames.

Keywords

Fusarium incarnatum, Bioinformatics Tools, Nucleotides, Atomic Study, Amino Acids, Frame Analysis.

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M. Mariyappan ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN

Abstract

The bacterial organisms were isolated from drinking water (Bacillus, Borchothrix, Clavibacter sp, Ancylobacter sp, and Brevi bacterium). Selected Indian medicinal plants Cardiospermum halicacabum and Melothria heterophylla were selected for antibacterial studies. The solvents used for the extraction of plant ischolar_mains were n- butanol, ethyl acetate and distilled water. The invitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Postive bacteria was Bacillus sp, Brevibacterium sp, and the most susceptible Gram-negative bacteria was Borchothrix sp, Clavibacter sp, and Ancylobacter sp. The extracts of plant Cardiospermum halicacabum and Melothria heterophylla inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that Cardiospermum halicacabum and Melothria heterophylla could be used in treating diseases caused by the test organisms. The results are discussed in detail.

Keywords

Medicinal Plants, Antibacterial Activity, Aqueous Extract, N-Butanol Excretory Ethyalcetate Extract.

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V. Saranya ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi, Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN

Abstract

Investigation on the cultivation of milky mushroom, Calocybe indica was conducted to find out the growth and yield performance on different substrates. The yield of fresh mushroom obtained from paddy straw, teak leaves and sugarcane trash substrates were 1140, 745 and 570 g/kg respectively. The nutritional significance of Calocybe indica cultivated on different substrates was assessed in terms of protein, carbohydrate, amino acids and lipids. The cellulolytic activity and coir pith compost was also tested using Calocybe indica. The present result suggest, the lignocellulosic substrate like paddy straw, teak leaves and sugarcane trash shown great potential for use as a raw material since this substrate provides an economically acceptable production for Calocybe indica.

Keywords

Calocybe indica, Sugarcane Trash, Teak Leaves, Composting of Coir Pith.

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P. Madhanraj ¹, N. Nadimuthu ², A. Panneerselvam ³

Affiliations
1 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur, Tamil Nadu, IN
2 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
3 P.G. and Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt.), Tamilnadu, IN

Abstract

A total number of 325 colonies were isolated by plating method from the soils of Casurina bioshield plantation at Naluvedhapathy (Nagapattinam Dt.), along south east coast of India. There were 19 species belonged to 4 genera with Aspergillus as the dominant genus, represented by more number of species (14 species), followed by Fusarium and Trichoderma (2 species each). Population density varied from 9.7 to 17.7 CFU/g and the species diversity from 6 to 10 species during different stations. Physico-chemical analyses of the soils revealed the following features: pH 7.9 to 8.8, electrical conductivity 1.06 to 1.31 dSm^-1, cation exchange capacity 8.67 to 10.11 c.mol proton⁺/kg, organic carbon 0.04 to 0.21%, available nitrogen 0.011 to 0.022%, available phosphorus 0.002 to 0.005%, available potassium 0.013 to 0.051 ppm, available zinc 0.47 to 0.57 ppm, available iron 2.13 to 2.90 ppm, available copper 0.22 to 0.31 ppm, available manganese 1.38 to 1.53 ppm, calcium 3.1 to 5.1 mg/kg, magnesium 3.2 to 4.1 mg/kg, sodium 0.23 to 0.79 mg/kg and potassium 0.02 to 0.08 mg/kg, during different seasons.

Keywords

Soil Fungi, Bioshield Plantation, Soil Characteristics, Mycoecology.

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G. Senthilkumar ¹, P. Madhanraj ¹, A. Panneerselvam ¹

Affiliations
1 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt), Tamil Nadu, IN

Abstract

Totally 42 species belonged to 20 genera were recorded. A preliminary screening of all the species isolated from soils were made for antifungal (antagonistic) activity against Fusarium oxysporum, a known soil borne fungal pathogen. Among the species tested the Trichoderma harzianum inhibited the pathogenic fungus to the maximum both in dual culture and in food poisoning technique. Gas chromatography mass spectrum analysis of acetonitrile extract of the filtrate of T. harzianum revealed the presence of six compounds represents six major peaks. The peaks correspond with diethyl phthalate, tetradecanoic acid 9,12-octadecadienoic acid (z,z), oleic acid, 1, 2-benzene di oxylic acid, diisooctyl ester and squalene.

Keywords

Fungal Extract, Compounds, GC-MS.

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