Asian Journal of Pharmaceutical Analysis

Krishna V. Prajapati ¹, Hasumati A. Raj ¹, Vineet C. Jain ¹

Affiliations
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Mesalazine and Rifaximin in synthetic mixture using Simultaneous Equation Method. The absorbance was measured at 328 nm for Mesalazine and 292nm for Rifaximin and calibration curves were plotted as absorbance versus concentration, respectively. The method was found to be linear (r²> 0.999) in the range of10-50 μg/ml for Mesalazine at 328nm. The linear correlation was obtained (r²> 0.999) in the range of 10-50 μg/ml for Rifaximin at 292nm. The limit of determination (LOD) was 0.215μg/ml and 0.214μg/ml for Mesalazine and Rifaximin respectively. The limit of quantification (LOQ) was 0.652μg/ml and 0.648 μg/ml for Mesalazine and Rifaximin respectively. The accuracy of this method was evaluated by recovery studies and good recovery results were obtained greater than 99%.The method was successfully applied for simultaneous determination of Mesalazine and Rifaximin in binary mixture.

Keywords

Mesalazine, Rifaximin, Simultaneous Estimation, Simultaneous Equation Method.

Full Text

     

Pranali S. Sisode ¹, Hasumati A. Raj ¹, Vineet C. Jain ²

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN
2 Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Saxagliptin Hydrochloride and Glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin Hydrochloride showed zero crossing point at 315.00nm while Glibenclamide showed zero crossing point at 229.40nm. The dA/dλ was measured at 229.40nm for Saxagliptin Hydrochloride and 315.00nm for Glibenclamide and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25μg/ml for Saxagliptin Hydrochloride at 229.40nm. The linear correlation was obtained (r2>0.9994) in the range of 5-25 μg/ml for Glibenclamide at 315.00nm. The limit of determination was 0.243μg/ml and 0.317μg/ml for Saxagliptin Hydrochloride and Glibenclamide, respectively. The limit of quantification was 0.738μg/ml and 0.960μg/ml for Saxagliptin Hydrochloride and Glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in binary mixture.

Keywords

Saxagliptin Hydrochloride, Glibenclamide, First Derivative Method, Spectroscopic Method.

Full Text

     

Priyanka P. Atodariya ¹, Hasumati A. Raj ¹, Vineet C. Jain ¹

Affiliations
1 Shree Dhanvantary Pharmacy Collage, Kim, Surat, Gujarat, IN

Abstract

Two simple spectroscopic methods have been developed for simultaneous estimation of Lamotrigine and Clozapine in synthetic mixture. Simultaneous equation method involves the measurement of absorption at two wavelengths 308 nm (λmax for Lamotrigine) and 259.60 nm (λmax for Clozapine). The method was found linear between the range of 1-5 μg/ml for Lamotrigine and 6-30 μg/ml for Clozapine for method .The accuracy and precision was determined and validated statistically. Both the method showed good reproducibility and recovery with %RSD less than 1.The method was found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis for Lamotrigine and Clozapine in bulk and combined dosage form.

Keywords

Lamotrigine, Clozapine, Simultaneous Equation Method.

Full Text

     

Farhana V. Buchiya ¹, Ashif I. Bhim ¹, Hasumati A. Raj ¹, Vineet C. Jain ²

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN
2 Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimationof Cilnidipine and Valsartan in synthetic mixture using Simultaneous Equation Method. The absorbance was measured at 240.00nm for Cilnidipine and 250.00nm for Valsartan and calibration curves were plotted as absorbance versus concentration, respectively. The method was found to be linear (r²>0.999) in the range of 2-10μg/ml for Cilnidipine at 240.00nm. The linear correlation was obtained (r²>0.999) in the range of 16-80μg/ml for Valsartan at 250.00nm. The limit of determination (LOD) was 0.07 μg/ml and 0.266μg/ml for Cilnidipine and Valsartan respectively. The limit of quantification (LOQ) was 0.22μg/ml and 0.808μg/ml for Cilnidipine and Valsartan respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99%.The method was successfully applied for simultaneous determination of Cilnidipine and Valsartan in binary mixture.

Keywords

Cilnidipine, Valsartan, Simultaneous Estimation, Simultaneous Equation Method.

Full Text