Abstract Views :222 |
PDF Views:2
Authors
Affiliations
1 Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 6, No 1 (2016), Pagination: 23-30
Abstract
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Amiodarone Hydrochloride and Ranolazine in synthetic mixture using Second order derivative zero-crossing method. Amiodarone HCl showed zero crossing point at 249.20 nm while Ranolazine showed zero crossing point at 263.20 nm. The dA/dλ was measured at 263.20 nm for Amiodarone HCl and 249.20 nm for Ranolazine and calibration curves were plotted as 2dA/2dλ versus concentration, respectively The linear correlation was obtained (r2>0.9996) in the range of 1-20 μg/ml for Amiodarone HCl at 263.20 nm. The method was found to be linear (r2>0.9996) in the range of 10-200 μg/ml for Ranolazine 249.20 nm. The limit of determination was 0.235μg/ml and 0.271μg/ml for Amiodarone HCl and Ranolazine, respectively. The limit of quantification was 0.712μg/ml and 0.823μg/ml for Amiodarone HCl and Ranolazine, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows Second order derivation zero crossing. The method was successfully applied for simultaneous determination of Amiodarone HCl and Ranolazine in binary mixture.
Keywords
Amiodarone HCl, Ranolazine, Second Derivative Method, Spectroscopic Method.
