Asian Journal of Pharmaceutical Analysis

Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

Two spectrophotometric methods are described for determination of Clarithromycin, Clindamycin and Cefixime in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbance at (666nm for clindamycin and cefixime or 678 nm for clarithromycin) and 510 nm respectively. The amount of bromine reacted corresponds to the amount of each drug. The effect's of acidity, bromate-bromide volume and time, on the absorption were studied. Calibration curves were linear over ranges of 3.2-16 μg.ml^-1 for Clarithromycin,1.6- 4.8 μg.ml^-1 for Clindamycin, 0.8-7.2 μg.ml^-1 for Cefixime in case of methylene blue and of 6.4-19.2 μg.ml^-1 for Clarithromycin, 0.8-4.0μg.ml^-1 for Clindamycin, 0.4-2 μg.ml^-1 for Cefixime in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Clarithromycin, Clindamycin, Cefixime, Methylene Blue and Methyl Orange.

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Sobhy M. El-Adl ¹, Mohamed El. Hossinny El. Sadek ¹, Marwa Hamdy Hassan ¹

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG

Abstract

An isocratic HPLC method had been developed for rapid simultaneous determinathion of three cephalosporines including cefepime HCl, cefoperazone, and cefotaxime Na in pure form or in pharmaceutical dosage form within less than 15 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 7.8 using triethyalamine (18:82 v/v) at ambient temperature. The flow rate was 1 ml/min and maximum absorption was measured at 254 nm.

Keywords

Cefepime, Cfotaxime, Cefoperazone , Phosphate Buffer and HPLC.

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