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Gandla, Kumaraswamy.
- RP-HPLC Method Development and Validation for Simultaneous Estimation of Lignocaine Hydrochloride and Clotrimazole Hydrochloride in Ear Drops
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Authors
Affiliations
1 Care College of Pharmacy, Oglapur (Vill), Damera (Mdl), Warangal, Telangna, IN
1 Care College of Pharmacy, Oglapur (Vill), Damera (Mdl), Warangal, Telangna, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 7, No 3 (2017), Pagination: 163-168Abstract
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Lignocaine and Clotrimazole, in its pure form as well as in ear drops. Chromatography was carried out on a Phenomenex C18 (4.6×250mm) 5μ column using a mixture of Methanol, Acetonitrile and potassium dihydrogen phosphate buffer (50:20:30) as the mobile phase at a flow rate of 1.2 ml/min, the detection was carried out at 220nm. The retention time of the Clotrimazole and Lignocaine was 2.266, 6.349min respectively. The method produce linear responses in the concentration range of 10-60μg/ml of Clotrimazole and 10-60μg/ml of Lignocaine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of pharmaceutical formulations.Keywords
Lignocaine HC1, Clotrimazole HC1, RP-HPLC, Validation.
References
- Anonymous ;https://en.wikipedia.org/wiki/Lignocaine Hcl.
- Anonymous URL:http://www.drugbank.ca/drugs/DB00863
- Anonymous URL: https://en.wikipedia.org/wiki/Clotrimazole
- United States Pharmacopoeia 30 and National Formaulary,(25)Asian Edition,The United States Pharmacopoeia Convention Inc.,U.S.A.Volume no 29(4):pp.1069.
- Indian Pharmacopoeia,Govy.of India “Ministry of health and family welfare”the controller andpublication ,Delhi(vol-II):pp.570.
- Meyer VR.Practical high performance liquid chromatography. 2nd ed. London: john wiley and sons; 1993.
- ICH Q2A, “validation of analytical methods, definitions and terminology”, ICH Harmonized tripartite guideline, (1999).ICH: Q2B, Analytical validation- methodology, 1996.
- Kashyap Shah1,Prasanna Ku. Pradhan2,Shreya R Shah2 Analytical Method Development and Validation for Simultaneous Estimation of Clotrimazole and Dexamethasone In Synthetic Mixture J Pharm Biomed Sci 2014;04(05):448-458.
- Singh Pradeep Kumar, Subas Chandra worked on Development and validation of a stability indicating RP-HPLC method for determination of Clotrimazole in orally disintegrating films. IJRPS 3(1), pg 57-66, 2013.
- Yadav A,Singh , Sharma DK, et al., worked on Development and validation of HPTLC method for the estimation of Clotrimazole hydrochloride in Bulk and Tablet dosage form. Jounal of Pharmaceutical Reasearch, vol 2,no.2, 61-65,2013.
- Kantariya B, Agola A et al., Development and Validation of a RPHPLC method for simultaneous estimation of Lignocaine hydrochloride and Dicyclomine hydrochloride in tablet dosage form. IJPRS, vol.2, issue.2, pg no. 258-267, year 2013.
- SN Meyyanathan, D Nagaswamy, N Krishnaveni, B Babu. Developed a RP-HPLC method for Simultaneous estimation of Clotrimazole and Lignocaine in Pharmaceutical Formulation. Indian Jounal of Health and Allied Sciences, vol-1, issue 2, pg no. 129-132, 2012.
- Snyder LR practical HPLC method development, 2nd edition. John Wiley and sons, New York, (1997), PP 180-182.
- Skoog D A, West D M, Holler FJ: Introduction of analytical chemistry. Sounder college of publishing, Harcourt Brace college publishers. (1994), PP 1-5.