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Rajput, Hrishikesh H.
- Capillary Electrophoresis: A Potential Tool for Separation and Analysis-Brief Review
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Authors
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1 Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), IN
1 Govt. College of Pharmacy, Osmanpura, Aurangabad 431 005, (M.S.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 7, No 4 (2017), Pagination: 243-250Abstract
Capillary electrophoresis (CE) is a potential analytical and separation technique for wide variety of analytes, ranging from micro to macromolecules of biological or chemical origin belongs to either inorganic or organic class possesses simple or complex molecules. CE technology has been valuable for the comprehensive determination of drug and process related impurities. CE is a family of electrokinetic separation methods performed in capillaries and in micro-fluidic channels rely on a high voltage electric field being applied over a solution which is held in a capillary tube. CE classified according to mode of separation on the basis of differences in charge, size and frictional force, offers fast separations with excellent efficiency. CE is one of favorite analytical tool for assay of pharmaceutical API including determination of drug related impurities, as it provides reliable result in minimum time period; also, it offers a high degree of automation and requires minimal sample preparation and. CE possess other versatile applications such as chiral, stoichiometric and bioanalysis of pharmaceutical API. The current review presents basic principles underlying different modes of CE, and their application in analysis of pharmaceutical analysis.Keywords
Capillary Electrophoresis, Non Aqueous Capillary Electrophoresis, Capillary Gel Electrophoresis, Capillary Isoelectric Focusing, Capillary Isotachophoresis.
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