Asian Journal of Pharmaceutical Analysis

Dibyajyoti Saha ¹, Swati Paul ¹

Affiliations
1 Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, BD

Abstract

The aerial part of Alpinia conchigera Griff (family: Zingiberaceae,) was studied to fix the parameters for pharmacognostical standards. The results of organoleptic study offer a scientific basis for the use of Alpinia conchigera Griff which possess characters like green colour, characteristic odour, mucilaginous and slightly bitter taste. The fluorescence analysis under visible UV light and under UV light by treatment with different chemical reagents showed different colour changes. These created an interest to test the possible phytochemical activity of the plant. In the screening process of Alpinia conchigera Griff indicate the presence of Reducing sugars, Steroids, Alkaloids, Flavonoids, Saponins and Gums. The Pharmacognostic study was performed by using standard procedure. Further, detailed studies are needed to know whether in-vivo administration of the extracts is beneficial for patients.

Keywords

Organoleptic, Fluorescence, Pharmacognostic Study.

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Dibyajyoti Saha ¹, Ankit Tamrakar ¹

Affiliations
1 School of Pharmacy, Chouksey Engineering College, Lal Khadan, Masturi Road, Bilaspur-495004, C.G., IN

Abstract

Protein mass spectrometry refers to the application of mass spectrometry to study the proteins and nowadays it is introduced in proteomies science as well as pharmaceutical biotechnology. Mass spectrometry is an important emerging method for ionization of whole proteins are electrospray ionization (ESI) and matrix assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as "top-down" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence this latter approach (also called "bottom up" proteomies) uses identification at the peptide level to infere the existence of proteins.

Keywords

Mass Spectrometry, Proteins, Peptides, High Performance Liquid Chromatography, Quantization.

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Dibyajyoti Saha ¹, Mayukh Jana ², Supradip Mandal ²

Affiliations
1 School of Pharmacy, Chouksey Engineering College, Lal Khadan, Masturi Road, Bilaspur-495004, C.G., IN
2 Bharat Technology, Banitabla, Uluberia, Howrah-711316, IN

Abstract

The molecular pharmacology involving aspects of biological, medical and pharmaceutical sciences. It is concerned with invention, discovery, design, identification and preparation of biologically active compounds, the study of their metabolism, the interpretation of their mode of action at the molecular level. The physiology based drug discovery follows physiological readouts and by contrast, the road of target based drugs discovery begins with identifying the function of a possible therapeutic target and its role in disease. The validation of a molecular target in vitro usually proceeds the validation of a therapeutic concept in vivo; together this defines its clinical potential. The present article gives disease mechanisms of target discovery and outline of target validation with its pathways.

Keywords

Target Discovery, Target Validation, Molecular Target, Disease Genes, Molecular Pharmacology.

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Dibyajyoti Saha ¹

Affiliations
1 School of Pharmacy, Chouksey Engineering College, Lal Khadan, Masturi Road, Bilaspur-495004, C.G., IN

Abstract

The mass spectrometry technique deals with a beam of electrons which produce an ionic molecule or ionic fragments of the original species. The resulting assortment of charged particle is then separated than according to their masses. The spectrum produced, known as mass spectrum is record information regarding various masses produced and their relative abundance. This article highlights potential approaches and theoretical aspects of mass spectrometry with its basic design.

Keywords

Mass Spectrometry, Ionized Fragments, Mass Analyzer, Gas Chromatography, Ion Mobility Spectrometry.

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