Asian Journal of Pharmaceutical Analysis

Audumbar Digambar Mali ¹

Affiliations
1 Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, IN

Abstract

Derivative spectrophotometry offers a useful approach for the analysis of drugs in multi-component formulation. In this study a second order derivative spectrophotometric method is applied for the simultaneous determination of Carvedilol and Hydrochlorothiazide in tablet dosage form. The measurements were carried out at wavelengths of 292 and 272 nm for Carvedilol and Hydrochlorothiazide respectively. The method was found to be linear (r²=0.999) in the range of 5-25 μg/ml for Carvedilol in the presence of 20 μg/ml of Hydrochlorothiazide at 292 nm. The linear correlation (r²=0.999) was obtained in the range of 5-25 μg/ml for Hydrochlorothiazide in the presence of 20 μg/ml of Carvedilol at 272 nm. The method was successfully used for simultaneous determination of Carvedilol and Hydrochlorothiazide in tablet dosage form without any interference from excipients and prior separation.

Keywords

Carvedilol, Hydrochlorothiazide, Uv Visible Spectrophotometry, Method Validation, Accuracy, Pricission, Second Order Derivative Method.

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Audumbar Digambar Mali ¹, Patil Manoj kumar ¹

Affiliations
1 Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, IN

Abstract

Simple, fast and reliable spectrophotometric methods were developed for determination of Ranitidine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the first order Derivative Area under Curve method values measured at 329-335nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ranitidine using 2-10μg/ml (r²=0.999) for first order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ranitidine in pharmaceutical formulations.

Keywords

Ranitidine, First Order Derivative, Area under Curve (AUC).

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