Asian Journal of Pharmaceutical Analysis

Akhilesh Gupta ¹, Vimal Yadav ¹, Jaydeep S. Yadav ¹, Swati Rawat ²

Affiliations
1 Kunwar Haribansh Singh College of Pharmacy, Jaunpur (U.P.), IN
2 Shri Bhagwan College of Pharmacy, Aurangabad (M.S.), IN

Abstract

Doxofylline is chemically designated as 7-(1, 3 dioxolone-2-yl methyl) theophylline. Presence of a dioxolane group in position C-7 differentiates it from theophylline. It is a new antibronchospastic drug recently introduced in therapy with pharmacological properties like theophylline; a potent adenosine receptor antagonist. doxofylline do not affect gastric acid secretion; either invivo or in-vitro; unlike theophylline. The lack of side effects with doxofylline indicates that the drug can be used safely and effectively. Some analytical methods for quantitative determination of doxofylline in pharmaceutical formulations like UV-Spectrophotometry, HPLC and LC-MS are reported. The present review deals with the various analytical methods reported as well as adopted for the estimation of doxofylline.

Keywords

Doxofylline, UV, HPLC, LC-MS.

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S. Rawat ¹, Akhilesh Gupta ²

Affiliations
1 Shri Bhagwan College of Pharmacy, Aurangabad (M.S.), IN
2 Kunwar Haribansh Singh College of Pharmacy, Jaunpur (U.P), IN

Abstract

A simple accurate spectrophotometric method has been developed for simultaneous estimation of nimesulide and diclofenac sodium. The method involves solving of simultaneous equation based on measurement of absorbance at two wavelengths i.e., at 276 nm and 395 nm.

Keywords

Nimesulide, Diclofenac Sodium, Simultaneous Equation and UV Spectrophotometry.

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Akhilesh Gupta ¹, Swati Rawat ², Arun Pandey ³

Affiliations
1 Kunwar Haribansh Singh College of Pharmacy, Jaunpur (U.P.), IN
2 Shri Bhagwan College of Pharmacy, Aurangabad (M.S.), IN
3 Alkem Laboratories, Mumbai, IN

Abstract

A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and photolytic degradation product. The method showed a linear response for concentrations in the range of 1-200 g/ml using acetonitrile: formic acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/min and retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The acid degradation product as well as pathway was characterized by LC-MS/MS.

Keywords

Doxofylline, RP-HPLC, LC-MS/MS, Degradation Studies.

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Akhilesh Gupta ¹, Swati Rawat ², Mayuri Gandhi ³, Jaydeep Singh Yadav ¹

Affiliations
1 Kunwar Haribansh Singh College of Pharmacy, Jaunpur (U.P.), IN
2 Shri Bhagwan College of Pharmacy, Aurangabad (M.S.), IN
3 IIT, Mumbai (M.S.), IN

Abstract

A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and acid degradation product. The method showed a linear response for concentrations in the range of 1-200 μg/ml using acetonitrile: formic Acid (90: 10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/min and retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The acid degradation product as well as pathway was characterized by LC-MS/MS.

Keywords

Doxofylline, RP-HPLC, LC-MS/MS, Degradation Studies.

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Akhilesh Gupta ¹, Jaydeep Singh Yadav ¹, Swati Rawat ², Mayuri Gandhi ³

Affiliations
1 Kunwar Haribansh Singh College of Pharmacy, Jaunpur (U.P.), IN
2 Shri Bhagwan College of Pharmacy, Aurangabad (M.S.), IN
3 IIT Mumbai (M.S), IN

Abstract

A simple, rapid and accurate RP-HPLC method was developed for the determination of doxofylline and photolytic degradation product. The method showed a linear response for concentrations in the range of 1-200 μg/ml using acetonitrile: formic acid (90:10); pH-3.0 as the mobile phase with detection at 274 nm and a flow rate of 1 ml/min and retention time 2.9 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, forced degradation, solution stability and selectivity. Quantitative and recovery studies of the dosage form were also carried out and analyzed; the % RSD from recovery studies was found to be less than 1. Due to simplicity, rapidity and accuracy of the method, we believe that the method will be useful for routine quality control analysis. The hydrolytic degradation product as well as pathway was characterized by LC-MS/MS.

Keywords

Doxofylline, RP-HPLC, LC-MS/MS, Degradation Studies.

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