Asian Journal of Pharmaceutical Analysis

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HPLC Determination of Three Cephalosporins (Cefepime, Cefotriaxone and Cefotaxime) in Their Bulk and Dosage Forms


Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
2 Department of Pharmaceutical Chemistry, Al-Azhar University, Assuit, Egypt
     

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An isocratic HPLC method had been developed for rapid simultaneous separation and determination of three ceohalosporins including Cefepime, Cefotriaxone and Cefotaxime in pure form or in presence of some impurities within 8 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase was studied. Beer's law was obeyed in the range of 1-70 μg/ml for all drugs. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and was validated when obtained results were compared with reference methods.

Keywords

Cefepime, Cefotriaxone, Cefotaxime, Hypersil Gold, Mobile Phase.
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  • HPLC Determination of Three Cephalosporins (Cefepime, Cefotriaxone and Cefotaxime) in Their Bulk and Dosage Forms

Abstract Views: 232  |  PDF Views: 1

Authors

Sobhy M. El-Adl
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Abdallah A. El-Shanawany
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Lobna M. Abdel-Aziz
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Ali F. Hassan
Department of Pharmaceutical Chemistry, Al-Azhar University, Assuit, Egypt

Abstract


An isocratic HPLC method had been developed for rapid simultaneous separation and determination of three ceohalosporins including Cefepime, Cefotriaxone and Cefotaxime in pure form or in presence of some impurities within 8 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase was studied. Beer's law was obeyed in the range of 1-70 μg/ml for all drugs. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and was validated when obtained results were compared with reference methods.

Keywords


Cefepime, Cefotriaxone, Cefotaxime, Hypersil Gold, Mobile Phase.