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HPLC Determination of Cefepime, Cefotaxime and Cefoperazone in Bulk and Dosage Forms


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1 Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
     

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An isocratic HPLC method had been developed for rapid simultaneous determinathion of three cephalosporines including cefepime HCl, cefoperazone, and cefotaxime Na in pure form or in pharmaceutical dosage form within less than 15 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 7.8 using triethyalamine (18:82 v/v) at ambient temperature. The flow rate was 1 ml/min and maximum absorption was measured at 254 nm.

Keywords

Cefepime, Cfotaxime, Cefoperazone , Phosphate Buffer and HPLC.
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  • HPLC Determination of Cefepime, Cefotaxime and Cefoperazone in Bulk and Dosage Forms

Abstract Views: 99  |  PDF Views: 2

Authors

Sobhy M. El-Adl
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Mohamed El. Hossinny El. Sadek
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Marwa Hamdy Hassan
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt

Abstract


An isocratic HPLC method had been developed for rapid simultaneous determinathion of three cephalosporines including cefepime HCl, cefoperazone, and cefotaxime Na in pure form or in pharmaceutical dosage form within less than 15 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 7.8 using triethyalamine (18:82 v/v) at ambient temperature. The flow rate was 1 ml/min and maximum absorption was measured at 254 nm.

Keywords


Cefepime, Cfotaxime, Cefoperazone , Phosphate Buffer and HPLC.