Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Development and Validation of RP-HPLC for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium Tablets


Affiliations
1 Jawaharlal Nehru Technological University Kakinada, Kakinada -533003.A.P., India
2 Department of Pharmaceutical analysis & QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad, 500097, Telangana, India
     

   Subscribe/Renew Journal


The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium tablets .The chromatographic separation was achieved on a Inertsil C18 ODS(4.6 x 250mm, 5􀀀m) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins. The retention time of Dicloxacillin Sodium and Cefpodoxime Proxetil were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords

RP-HPLC, Cefpodoxime Proxetil, Dicloxacillin Sodium, Tablet Dosage Forms, RP-HPLC Method.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 122

PDF Views: 1




  • Development and Validation of RP-HPLC for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium Tablets

Abstract Views: 122  |  PDF Views: 1

Authors

G. Kumaraswamy
Jawaharlal Nehru Technological University Kakinada, Kakinada -533003.A.P., India
M. A. Zeeshan Hamza
Department of Pharmaceutical analysis & QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad, 500097, Telangana, India
R. Suthakaran
Department of Pharmaceutical analysis & QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad, 500097, Telangana, India

Abstract


The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium tablets .The chromatographic separation was achieved on a Inertsil C18 ODS(4.6 x 250mm, 5􀀀m) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins. The retention time of Dicloxacillin Sodium and Cefpodoxime Proxetil were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords


RP-HPLC, Cefpodoxime Proxetil, Dicloxacillin Sodium, Tablet Dosage Forms, RP-HPLC Method.