The Asian Journal of Experimental Chemistry

M. Kiranmai Reddy ¹, G. Himavathi ², S. Manga ³, T. Srinivas ³

Affiliations
1 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam (A.P.), IN
2 Department of Biotechnology, GITAM Institute of Technology, GITAM University, Visakhapatnam (A.P.), IN
3 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam (A.P.), IN

Abstract

The air consists of several bioallergens like spores of bacteria, fungi, organic dust and pollen grains which can cause severe allergic reactions in humans. To estimate the bioallergens, a study of air micro flora was conducted in different locations in Visakhapatnam. The fungal density in the air varied from 0.9x10¹ to 4.3 x10³. In the present study twenty two fungal species were reported. The most common fungi identified were Aspergillus, Cladosporium, Alternaria, Penicillum, Curvularia, Mucor and Rhizopus. Aspergillus strains were present in alarming levels followed by Cladosporium, Alternaria and Curvularia. Fungal spores are known to be potential aeroallergen and could well be a health hazard to all people traveling regularly in these areas.

Keywords

Bioallergens, Air, Fungi, Health Hazard.

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G. Himavathi ¹, M. Kiranmai Reddy ²

Affiliations
1 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam (A.P.), IN
2 Department of Chemistry, GIT Institute of Technology, GITAM University, Visakhapatnam (A.P.), IN

Abstract

Three simple accurate visible sepectrophotometric methods (A.B and C) have been developed for the estimation of Drotaverine (DRT) in bulk and pharmaceutical formulations. The estimation was done based on its complex formation with alkaloids using spectrophotometric methods. Drotaverine forms a molecular complex with SNP (Sodium Nitro Prusside) in method-A, CTC (Cobalt thiocyanate) in method B and DDQ (2,3-dichloro-dicyano-1,4-benzoquinone) in method C during its quantitative precipitation. The absorbance of nitrobenzene layer was measured at the respective wavelength of maximum absorbance against the reagent blank. To determine Drotaverine colour reaction was used in addition to precipitation reaction. They are based on the colour formation with either unreacted precipitant of the filtrate or released precipitant from the molecular complex. All the variables have been optimized. The proposed methods are validated statistically. Recovery studies were carried out by standard addition method.

Keywords

Drotaverine, SNP, CTC, DDQ and Spectrophotometer.

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