The Asian Journal of Experimental Chemistry

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Method Development and Validation for the Simultaneous Estimation of Aceclofenac, Paracetamol and Chlorzozazone in Pharmaceutical Dosage Forms by High Performance Thin Layer Chromatography


Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai (T.N.), India
2 Department of Medical Chemistry, J.S.S. University, Ootacamund (T.N.), India
3 Department of Pharmacognosy, Bapatala College of Pharmacy, Bapatla (A.P.), India
4 Rishiraj College of Pharmacy, Indore (M.P.), India
     

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A simple, rapid, sensitive and highly precise High Performance Thin Layer Chromatographic Method has been developed for the estimation of Paracetamol, Aceclofenac and Chlorzoxazone in tablets. HPTLC was performed on CAMAG LINOMAT IV, TLC Scanner Version 3.20, using toluene, ethyl acetate and glacial acetic acid (17.5:10:0.5 v/v) as mobile phase. The Chromatogram was developed in CAMAG twin trough glass containing mobile phase. The TLC plates were scanned at 271 nm in schimadzu dual wavelength scanner, and Rf value of Paracetamol, Aceclofenac and Chlorzoxazone was found to be 0.12, 0.29 and 0.72, respectively. The linearity of Paracetamol, Aceclofenac and Chlorzoxazone shows a correlation coefficient of 0.9995, 0.9991, and 0.9997, respectively. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters.

Keywords

Paracetamol, Aceclofenac, Chlorzoxazone, Toluene, Ethyl Acetate, Glacial Acetic Acid, HPTLC, Validation.
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  • Method Development and Validation for the Simultaneous Estimation of Aceclofenac, Paracetamol and Chlorzozazone in Pharmaceutical Dosage Forms by High Performance Thin Layer Chromatography

Abstract Views: 193  |  PDF Views: 2

Authors

T. Venkatesh Karthikeyan
Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai (T.N.), India
V. Vaithyalingam
Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai (T.N.), India
G. Yuvaraj
Department of Medical Chemistry, J.S.S. University, Ootacamund (T.N.), India
A. Ravi Kumar
Department of Pharmacognosy, Bapatala College of Pharmacy, Bapatla (A.P.), India
R. K. Nema
Rishiraj College of Pharmacy, Indore (M.P.), India

Abstract


A simple, rapid, sensitive and highly precise High Performance Thin Layer Chromatographic Method has been developed for the estimation of Paracetamol, Aceclofenac and Chlorzoxazone in tablets. HPTLC was performed on CAMAG LINOMAT IV, TLC Scanner Version 3.20, using toluene, ethyl acetate and glacial acetic acid (17.5:10:0.5 v/v) as mobile phase. The Chromatogram was developed in CAMAG twin trough glass containing mobile phase. The TLC plates were scanned at 271 nm in schimadzu dual wavelength scanner, and Rf value of Paracetamol, Aceclofenac and Chlorzoxazone was found to be 0.12, 0.29 and 0.72, respectively. The linearity of Paracetamol, Aceclofenac and Chlorzoxazone shows a correlation coefficient of 0.9995, 0.9991, and 0.9997, respectively. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters.

Keywords


Paracetamol, Aceclofenac, Chlorzoxazone, Toluene, Ethyl Acetate, Glacial Acetic Acid, HPTLC, Validation.