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Upadhyay, Upashna
- Detection of Polymorphism of ATM Gene in Leukemia
Authors
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 100-105Abstract
The Ataxia telangiectasia mutated gene encodes the ATM protein, a key element in the DNA damage response (DDR) signalling pathway responsible for maintaining genomic integrity within the cell. In order to study the genetic changes of ATM gene in Lukaemia, we collected the blood samples of healthy persons for control and leukaemia persons for disease samples. We isolated DNA and performed PCR to analyse by Gene sequencing. As a result we found less number of sequencing result in nucleotide BLAST due to which we are unable to detect the compared result of mutation. Larger and/or combined association studies are needed to conclude the result.
Keywords
ATM Gene, Gel Eelectrophoresis, Polymerase Chain Reaction, DNA Sequencing.
- Isolation and Optimization of Lipase Producing Bacteria from Oil Contamination Soils
Authors
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 131-135Abstract
Lipolytic bacteria were isolated from oil contaminated soil and grown on glycerol tri-butyrate media. The lipase activity is shown by reacting with various chemicals. Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Bacterial lipase producers were isolated from oil spilled soil from vegetable oil processing factories. The effect of incubation time, medium pH, temperature, agitation, inoculums concentration, carbon source and nitrogen source for the lipase production was studied. The lipase production was maximum at pH 7, temperature by the lipase producing bacteria Staphylococcus. Increased enzymatic production was obtained when the organisms were cultured in medium supplemented with 1per cent protease peptone by Staphylococcus. The results of the present study was to demonstrate that the micro-organism is ideal for extracellular lipase production at industrial level. Different media parameters were optimized for maximal enzyme production. Lipases activity shown on starch containing agar media at pH-7.0 and temperature at 370C for 24-48 h.