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Ismail, Syed
- Impact of Integrated Nutrient Management Practices on Yield, Juice Quality and Nutrient Uptake in Sweet Sorghum [Sorghum biocolor (L.) Moench] Grown on Vertisol
Authors
1 Department of Soil Science and Agricultural Chemistry, Marathwada Agricultural University, Parbhani (M.S.), IN
Source
Asian Journal of Bio Science, Vol 4, No 1 (2009), Pagination: 100-102Abstract
A field experiment was conducted on Typic Haplustert at Research Farm, Marathwada Agricultural University, Parbhani (M.S.), India in rainy season of 2005-06. The soil was slightly alkaline (pH 8.2) and low in available N (231 kg ha-1) and moderate in availability of P2O5 (15 kg ha-1) and high in K2O (472 kg ha-1) having DTPA extractable Zn and Fe 0.79 and 4.29 mg kg-1, respectively. The experiment was laid out in Randomized Block Design (RBD) with seven treatments replicated thrice. The sweet sorghum variety used was HES-04. Inorganic fertilizers were applied as per recommended dose of fertilizer and micronutrients as per treatment through chemical fertilizers. However, Azotobacter and phosphorus solubilizing bacteria (PSB) were used for seed treatment before sowing. Vermicompost was applied @ 2.5 Mg ha-1 as per treatment at the time of sowing. Green stalk and grain yield, chemical analysis, Juice extraction, total soluble solid (°Brix), reducing and non-reducing sugars were determined at physiological maturity using standard procedures. The grain and green stalk yield of sorghum was significantly improved by application of inorganic fertilizers @ 50% RDF+vermicompost and micronutrients (Zn and Fe) along with seed treatment of biofertlizers. Similarly, addition of micronutrients also has contributed in increasing yield of crop. Quality of sweet sorghum in terms of juice extraction (%), °brix, reducing and non-reducing sugar was also found to increase with different integrated management practices particularly with application of 50% RDF+vermicompost+biofertilizer along with micronutrients. Comparatively higher nutrient concentration of NPK in grain and stover was found in 50% RDF along with micronutrients and Fe and Zn in stover was found higher in same treatment.Keywords
Sweet Sorghum, Vermicompost, Biofertilizers, Micronutrients.
- PCR Amplification and Cloning of Proteinase Inhibitor Gene in Cotton (Gossypium species)
Authors
1 Department of Agril.Biotechnology, Marathwada Agricultural University, Parbhani - 431 402 (M.S.), IN
2 Central Institute For Cotton Research (ICAR), Nagpur (M.S.), IN
Source
Asian Journal of Bio Science, Vol 1, No 2 (2006), Pagination: 140-143Abstract
The main objective of the work was to see presence of the three different Proteinase inhibitor genes of soybean Viz. Kti3, CI-II and PI-IV genes in cotton by synthesizing specific primer from already published data. DNA was extracted from different cotton spp. and was used for PCR amplification by the different specific primers for the Kti3, CI-II and PI-IV genes. Results have shown expected fragments of 651 bp for Kti3 gene and 250 bp for CI-II and PI-IV genes. These PCR fragments were were electroeluted from the Agarose gels and then used for cloning. These genes were then cloned in plasmid vector. Selection of transformed colony was done on the basis of Blue-White screnning. Plasmid was isolated and results were reconfirmed by doing PCR amplification of the Kti3, CI-II and PI-IV genes using specific primers. Results showed that the expected fragments were successfully cloned in the plasmid.