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Shinde, Snehal J.
- Identification and Utilization of Actinobacteria for Biocontrol of Rice Sheath Blight Pathogen, Rhizoctonia solani
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Authors
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1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), IN
1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 9, No 2 (2014), Pagination: 227-233Abstract
Sheath blight of rice is an important soil-borne fungal disease caused by Rhizoctonia solani. Isolation and exploitation of bioagents associated with rice offers a great opportunity for sustainable management of rice diseases. Actinomycetes were isolated from rice rhizosphere and isolates were putatively selected based on characteristic colony morphology. 16S rDNA and ARDRA confirmed their identification as actinobacteria. Isolates belonged either to Streptomyces spp. or Actinopolymorpha spp. Under in vitro condition, isolates IABT-A1, IABTA2, IABT-A3, IABT-A6, IABT-A7, IABT-A8 and IABT-A9 showed 98-100 per cent inhibition of Rhizoctonia solani, rice sheath blight pathogen. Further, five isolates were evaluated under glass house condition and the potent actinobacteria against rice sheath blight was identified. Among these isolates, IABT-A7 (Actinopoymorpha spp.) was most effective and application as seed treatment, soil and foliar spray was found most promising in terms of disease reduction and plant growth promotion. Actinopoymorpha spp. (IABT-A7) enhanced plant height, ischolar_main length and ischolar_main biomass in addition to reduced sheath blight infection in rice. This result indicates the role of actinobacteria isolate IABT-A7, as the probable stimulator of ISR (Induced Systemic Resistance) signaling pathway involved in plant disease resistance.Keywords
Rice, Rhizoctonia solani, Actinomycete, Biocontrol, Sheath Blight
References
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- Antipathogenic Potentiality of Fluorescent Pseudomonads for the Management of Rice Sheath Blight Pathogen, Rhizoctonia solani
Abstract Views :521 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), IN
1 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 9, No 2 (2014), Pagination: 255-260Abstract
Sheath blight (ShB) of rice is an important soil-borne fungal disease caused by Rhizoctonia solani (Kuhn) causing up to 40 per cent of yield losses annually. The present investigation was aimed to study the effect of Fluorescent pseudomonads on sheath blight management in rice and impact on plant growth. Fifteen different pseudomonad isolates were evaluated for their antagonistic activity against Rhizoctonia solani isolates under in vitro condition. Per cent inhibition of mycelial growth of R. solani by pseudomonads ranged from 74-100. All the isolates showed antagonism against the pathogen. Five strains, 12, 20, 19, soy2 and soy6 were found potent with 87-100 per cent inhibition of mycelial growth. They were further evaluated in greenhouse as seed treatment, soil application, foliar spraying and combined (seed+soil+foliar application) treatment for sheath blight control. Fluorescent pseudomonad isolate 19 was found potent and promising as it reduced the disease to the maximum extent and stimulated plant and ischolar_main growth.Keywords
Rice, Rhizoctonia solani, Sheath Blight, Fluorescent Pseudomonads, Biocontrol.References
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