Asian Journal of Bio Science

Shruti Shukla ¹, O. P. Verma ¹

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Science, ALLAHABAD (U.P.), IN

Abstract

Microbial alkaline proteases are among the important hydrolytic enzyme and have been used extensively since the advent of enzymology. Bacterial extracellular alkaline proteases are of great importance due to its wide spectrum applications in detergent industries, bioremediation, food industries, and leather processing and bio-film degradation. From the various niches eighteen isolates were screened for alkaline protease production, out of which four isolates showed efficient alkaline protease production. Out of four bacterial species one of the isolate i.e. N. flavescence showed significant enzyme activity. Optimization of the pH and temperature conditions for enzyme production was determined and was found to be 7.0 and 60°C, respectively. Optimization of carbon, nitrogen sources and metal ions for enzyme production were determined and was found to be 4.64U/ml for sucrose, 0.91U/ ml for gelatin and 0.21U/ml for zinc chloride for the isolates. The yield of alkaline protease was inhibited by copper sulphate. Enzyme activity was assayed using tyrosine-casein method. The purified enzyme preparations of having enzyme activity 0.26U/ ml was also excellent in destaining of ink colour. The molecular weight of different bands of alkaline protease fromN. flavescence ranged from 25kD to 83kD.

Keywords

Neisseria flavescence, Alkaline protease, SDS, Sucrose, Gelatin, Zinc chloride, Copper sulphate.

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Suman Tiwari ¹, O. P. Verma ¹, Shruti Shukla ¹

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN

Abstract

β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60^ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.

Keywords

Hordeum vulgare, β-Glucosidase, Purification, Characterization.

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Sangeeta Kumari ¹, K. Sahu ¹, A. Mehta ¹, Shruti Shukla ¹

Affiliations
1 Department of Botany, Lab of Microbiology, Dr. H. S. Gour Univ., Sagar (M.P.), IN

Abstract

The aim of this study was to determine physicochemical parameters of Sagar lake. This study was undertaken in Sagar lake, India, from January 2004 to June 2004. Samples were collected from three sampling sites and transported to the laboratory for analysis of physicochemical parameter of interest was carried out following the standard methods for water and waste water. The CD at 0.05 of DO nitrate, BOD, COD, phosphate, CO₂ alkalinity and chloride were 7.288, 28.22, 27.32, 56.17, 0.219, 2.55, 101.17, 278.26, respectively. From the parameters analyzed in this study, it is evident that Sagar district residents threatened the ecological integrity of Sagar lake by discharging organic waste into it without any form of treatment. Consequently, aquatic weeds now cover the major portion of the lake area leading to progressive eutrophication. The investigation shows that Sagar lake water is not safe for household purpose.

Keywords

Sagar Lake Water, Physico-Chemical Parameters.

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