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Authors
Affiliations
1 Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar (A.P.), IN
Source
Asian Journal of Bio Science, Vol 5, No 1 (2010), Pagination: 92-98
Abstract
Groundnut or peanut (Arachis hypogaea L.) is one of the principal economic crops of the world. The cotyledons, shoot apices, leaflets and embryo were selected as the explants to study the shoot regeneration system of groundnut from different explants of Kadiri 3. From the above set of experiments cotylydonary explants showed better response in shoot initiation medium (SIM), so that it was used in all the experiments on the optimization of regeneration system. The cotylydonary explants from mature seeds of peanut variety Kadiri 3 were cultured on different media formulations containing varying concentrations of BA and 2,4-D. Amongst the different media tested, MS containing 20 μM BA and 10 μM 2,4-D produced the highest frequency (73.3%). The explants bearing shoot buds were cut into two to four pieces and transferred on to shoot elongation medium, MS medium with 2 μM BA showed better response and the shoots were micropropagated on SEM through nodal explants for clonal multiplication and eventually ischolar_mained on MS medium containing 5 μM NAA where the other auxins didn’t show any promising results when compared with the NAA.
Keywords
Groundnut, Regeneration, Growth Harmones.
