Asian Journal of Bio Science

S. Devikrishna ¹, K. Kumutha ¹, P. Santhanakrishnan ¹, L. Srimathi Priya ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

Vesicular arbuscular mycorrhizal(VAM) fungi are a unique group of ubiquitous soil microorganism known to form symbiotic association with ischolar_mains of economically important crop plants. AM propagules such as isolated spores, sheared mycorrhizal ischolar_mains are virtually able to initiate AM symbiosis and establish the pre-symbiotic phase with the transformed ischolar_main. The spores were sterilized with 96 % ethyl alcohol and treated for 30, 60 and 90 seconds. Surface sterilization of AM spore viz., Glomus mosseae, G. intrardices and G. caledonium with 96 per cent ethyl alcohol for 60 sec exhibited higher germination per cent without affecting the viability of spores. Further cold treatment of spores at 4°C for a period of 30 days improved the spore germination to 90 % and 56.5 % in Glomus intraradices and Glomus caledonium, respectively, whereas Glomus mosseae, recorded maximum with 20 days of cold treatment. Though germination was observed higher with 30 days and cold treatment, stratification at 40C for a perod of 16-20 days resulted better germination of spores.

Keywords

AM Fungi, Sterilization, Spore Germination, Glomus.

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M. Iniyakumar ¹, A. Ramalakshmi ², P. Santhanakrishnan ²

Affiliations
1 Department of Civil Engineering, National University of Ireland, Galway, IE
2 Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

Crude glycerol, a by product of Jatropha biodiesel was used as carbon source for production of citric acid by Yarrowia lipolytica MTCC 35. Concentrations of crude glycerol ranging from 1 to 4 per cent were taken in buffered and unbuffered broth for the optimized production of citric acid by Yarrowia lipolytica MTCC 35. The amount of citric acid produced in buffered broth (11.2 gl^-1) was doubled when compared to unbufferd broth (5.2 gl^-1). Quantitative estimation through HPLC also confirmed the same.

Keywords

Citric Acid, Yarrowia lipolytica, Crude Glycerol-Biodiesel by Product.

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