Asian Journal of Bio Science

S. Vijayakumar ¹, K. Kumutha ¹, P. Santhana Krishnan ¹, H. Gopal ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

The laboratory experiment was conducted to investigate the effect of different concentrations of glucose ranges from 0.11 to 0.66 M and utilization pattern (7 days) was studied for lipid production in oleaginous yeast cultures. Lipid and biomass production was gradually increased in response to glucose concentration. In Rhodotorula glutinis, Rhodosporidium toruloides and Lipomyces starkeyi lipid content reached maximum as 4.55 g l^-1 (40.26 per cent), 4.25 g l^-1 (38.20 per cent) and 4.23 g l^-1 (38.10 per cent), respectively at 0.55 M of glucose concentration. Biomass content was also high as 11.30, 11.13 and 11.10 g l^-1, respectively at 0.55 M concentration. While studying the utilization pattern, consumption of glucose was started from the second day with small amount of lipid and biomass production and it was gradually increased on third and fourth days of fermentation period. Maximum amount of lipid (4.80, 4.64 and 4.61 g l^-1) and biomass (11.40, 11.26 g, 11.20 g l^-1) was recorded on fifth day of fermentation with the utilization of 0.5 M of glucose. Then decline in lipid production was observed on sixth and seventh days. At the end of fermentation Rhodotorula glutinis utilized 0.55 M of carbon and exhibited 4.56 g l^-1 of lipid and 11.30 g l^-1 of biomass.

Keywords

Oleaginous Yeast, Lipids, Biomass, Carbon Source, Glucose Utilization.

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S. Vijayakumar ¹, K. Kumutha ¹, P. Santhana Krishnan ¹, H. Gopal ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

The laboratory experiment was conducted to investigate the effect of different concentrations of glucose ranges from 0.11 to 0.66 M and utilization pattern (7 days) was studied for lipid production in oleaginous yeast cultures. Lipid and biomass production was gradually increased in response to glucose concentration. In Rhodotorula glutinis, Rhodosporidium toruloides and Lipomyces starkeyi lipid content reached maximum as 4.55 g l^-1 (40.26 per cent), 4.25 g l^-1 (38.20 per cent) and 4.23 g l^-1 (38.10 per cent), respectively at 0.55 M of glucose concentration. Biomass content was also high as 11.30, 11.13 and 11.10 g l^-1, respectively at 0.55 M concentration. While studying the utilization pattern, consumption of glucose was started from the second day with small amount of lipid and biomass production and it was gradually increased on third and fourth days of fermentation period. Maximum amount of lipid (4.80, 4.64 and 4.61 g l^-1) and biomass (11.40, 11.26 g, 11.20 g l^-1) was recorded on fifth day of fermentation with the utilization of 0.5 M of glucose. Then decline in lipid production was observed on sixth and seventh days. At the end of fermentation Rhodotorula glutinis utilized 0.55 M of carbon and exhibited 4.56 g l^-1 of lipid and 11.30 g l^-1 of biomass.

Keywords

Oleaginous Yeast, Lipids, Biomass, Carbon Source, Glucose Utilization.

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S. Vijaya Kumar ¹, K. Kumutha ¹, P. Santhana Krishnan ¹, H. Gopal ¹

Affiliations
1 Department of Agricultural Microbiology, Tamil Nadu Agriculture University, Coimbatore (T.N.), IN

Abstract

This study was undertaken to investigate the influence of nitrogen sources on lipid production by different yeast cultures. Among the four nitrogen sources, yeast extract and peptone showed maximum lipid production in biomass (22.85 and 20.03 per cent, respectively) and they were at par with each other, while ammonium sulphate (16.70 per cent) and sodium nitrate (13.34 per cent) exhibited poor lipid accumulation. Yeast extract containing broth supported to produce maximum lipid in Rhodotorula glutinis (23.82 per cent), followed by Rhodosporidium toruloides (22.53 per cent) and Lipomyces starkeyi (22.21 per cent). High amount of biomass as 10.20, 10.16 and 10.14 g l^-1 was also observed using yeast extract as nitrogen source, respectively of the above cultures. Further experiment on optimization of the concentrations ranging from 0.5 to 1.75 per cent revealed the production of higher lipids was under 0.75 per cent. Among the various concentrations evaluated, maximum lipid and biomass production was observed in Rhodotorula glutinis (31.3 per cent and 11.5 g l^-1), Rhodosporidium toruloides (29.72 per cent and 11.10 g l^-1) and Lipomyces starkeyi (28.50 per cent and 11.05 g l^-1), respectively at 0.75 per cent level. Reduction in biomass as well as lipid yield was observed when increase in concentrations of yeast extract beyond 1.0 per cent.

Keywords

Nitrogen, Oleaginous Yeast Cultures, Cell Biomass.

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