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Authors
Affiliations
1 Institute of BioScience, Ganpat University, Mehsana (Gujarat), IN
2 Central Salt & Marine Chemical Research Institute, Bhavnagar (Gujarat), IN
3 Department of Medicinal Crop, Anand Agricultural University, Anand (Gujarat), IN
4 Nutan Science College, Visnagar (GUJARAT), IN
Source
Asian Journal of Bio Science, Vol 6, No 1 (2011), Pagination: 51-58
Abstract
The geographical area of Karnataka is classified into ten agroclimatic zones. The first species of the genus Azotobacter named Azotobacter chroococcum, was isolated from the soil.The investigation was carried out to study the genetic variation of Azotobacter chrococcum occurs in the soils of the ten different agroclimatic zones of Karnataka. These isolates were characterized by using RAPD markers. A total of 103 bands were scored out of which 87 bands were found to be polymorphic (84.97%). Statistical analysis of RAPD data enabled the classification of 10 A. chroococcum isolates into three major groups. The RAPD banding pattern of these isolates could easily distinguish the isolates of different zones. In this the cluster analysis based on 103 RAPD bands revealed that the ten A. chroococcum isolates examined, clustered at a linkage distance of about 55 units on the dendrogram.
Keywords
Azotobacter chrococcum, Waksman No. 77 N-Free Medium, Rapd, Opb-12, Hind3 Marker, OPD-05.
