Asian Journal of Bio Science

Linu M. Salim ¹, Stephen Joseph ¹, M. S. Jisha ¹

Affiliations
1 School of Biosciences, Mahatma Gandhi University, P.D.Hills, Kottayam (Kerala), IN

Abstract

A pot culture experiment was carried out to assess the impact of novel phosphate solubilizing bacteria on yield parameters, nodulation and nutrient uptake of cowpea [Vigna unguiculata (L.) Walp.]. The experiment consists of nine treatments. Among them plants receiving phosphate solubilizing bacteria identified as Pseudomonas sp. and Acetobacter sp. amended with or without rock phosphate was superior over other treatments in all respects and were comparable with the treatment receiving Pseudomonas striata.

Keywords

Phosphate Solubilizing Bacteria, Vigna unguiculata, Rhizosphere.

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Stephen Joseph ¹, M. S. Jisha ¹

Affiliations
1 Department of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam (Kerala), IN

Abstract

Native Phosphate solubilizing bacteria (PSB) possessing the ability to solubilize insoluble inorganic phosphates were isolated from rhizosphere soils of crop plants. Eighty one potential PSBs thus obtained were quantitatively screened for phosphate solubilization. The amount of P solubilized for different bacteria varied between 11.38-72.97 mg/100 mL. Of these, two bacteria (PSB 12 and 73) found to be efficient phosphate solubilizers. These organisms were characterized on the basis of morphological, cultural and biochemical characteristics as Gluconacetobacter sp. and Burkholderia plantarii. The isolates were further tested for their tolerance to pesticides, Ekalux, Tagban, Sevin, Metacid and Hinosan. The two organisms showed remarkable difference in pesticide tolerance. Their minimum inhibitory concentration (MIC) values range between 48-3125 μg/mL. Quantitative estimation of soluble P in pesticide containing Pikovaskaya’s broth indicates that pesticides have an inhibitory effect on phosphate solubilizing activity.

Keywords

Pesticides, P Solubilization, Rhizosphere.

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