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Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak
(Haryana), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 91-94
Abstract
The strain improvement of developed Bacillus licheniformis SK7 was achieved with the combination of physical and chemical agents i.e., UV + NTG + EMS by gradually mutation and positive selection. In case of UV light 99 per cent killing for successful selection of mutants was 6 min and 10 cm distance from source, while in case of EMS and NTG the concentration found effective was 200 μM, 30 μM, respective for 15 min. The mutant Bacillus licheniformis SK7 SN 43 was successfully developed and found stable having higher production of protease (662 U/ml) under optimized medium and physical conditions of fermentation than the wild type Bacillus licheniformis SK7 (472 U/ml) under the same conditions of fermentation.
Keywords
Mutagenesis, NTG, UV Light, Strain Improvement, Protease.
