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Wable, K. J.
- Cryopreservation of Citrus reticulata Embryonic Axes by Vitrification Technique
Authors
1 Department of Agril. Biotechnology, Marathwada, Agriculture University Parbhani - 431 402 (M.S.), IN
Source
Asian Journal of Bio Science, Vol 1, No 2 (2006), Pagination: 112-113Abstract
The importance of loading and treatment with a vitrification solution on the survival of Citrus reticulata embryonic axes cryopreserved using vitrification technique (Derudde et al., 1990) were investigated in this paper. Loading treatment was carried out for 20 min. at 25 0C before chemical dehydration with 100 per cent PVS2 for 20 min. After treatment with PVS2 rapid immersion in liquid nitrogen, rapid rewarming, unloading in a 1.2 M sucrose solution for 20 min. Then the culture on solid medium with 0.3 M sucrose for 1 day and growth recovery for 4 weeks on standard medium, survival of C. reticulata embryonic axes reached 60 per cent following the process.Keywords
Citrus, Vitrification, Cryopreservation.
- PCR Amplification and Cloning of Proteinase Inhibitor Gene in Cotton (Gossypium species)
Authors
1 Department of Agril.Biotechnology, Marathwada Agricultural University, Parbhani - 431 402 (M.S.), IN
2 Central Institute For Cotton Research (ICAR), Nagpur (M.S.), IN
Source
Asian Journal of Bio Science, Vol 1, No 2 (2006), Pagination: 140-143Abstract
The main objective of the work was to see presence of the three different Proteinase inhibitor genes of soybean Viz. Kti3, CI-II and PI-IV genes in cotton by synthesizing specific primer from already published data. DNA was extracted from different cotton spp. and was used for PCR amplification by the different specific primers for the Kti3, CI-II and PI-IV genes. Results have shown expected fragments of 651 bp for Kti3 gene and 250 bp for CI-II and PI-IV genes. These PCR fragments were were electroeluted from the Agarose gels and then used for cloning. These genes were then cloned in plasmid vector. Selection of transformed colony was done on the basis of Blue-White screnning. Plasmid was isolated and results were reconfirmed by doing PCR amplification of the Kti3, CI-II and PI-IV genes using specific primers. Results showed that the expected fragments were successfully cloned in the plasmid.