Asian Journal of Bio Science

R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ²

Affiliations
1 Department of Biochemistry, NRI Medical College, China Kakani (A.P.), IN
2 Department of Chemistry, Kakatiya University, WARANGAL (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) from pigeon egg white and egg yolk have been isolated. The molecular weight of purified RCP of pigeon egg white and yolk has been found to be nearly same as that of previously isolated hen egg white and yolk RCPs. The amino acid composition of pigeon RCPs were observed to be almost similar to that of hen RCPs.

Keywords

Riboflavin Carrier Protein, Amino Acid Composition, Purification, Columba livia.

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R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ³

Affiliations
1 Department of Biochemistry, N. R. I. Medical College, CHINA KAKANI, (A.P.), IN
2 Department of Chemistry, Univ. Arts & Science College, Kakatiya University, Warangal, (A.P.), IN
3 Department of Chemistry, Kakatiya University, Warangal, (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) has been purified from both pigeon and hen egg- yolk by ion exchange chromatography and gel filtration techniques. Purity of pigeon egg- yolk and hen egg-yolk RCPs are determined by SDS-PAGE. Their structures are compared using Circular Dichroism (cd) Spectra and Fluorescence Spectra. For Pigeon egg-yolk RCP amino acid composition was analyzed. In the present study it is observed that the two proteins have similar secondary structure but they differ in their tertiary structures. The amino acid composition of pigeon egg-yolk RCP has close similarity with hen egg-yolk RCP. This study suggests the existence of some significant structural difference between RCPs from pigeon and hen.

Keywords

Riboflavin Carrier Protein, Protein Purification and Characterization.

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