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Patil, H. B.
- Pearl Millet Characterization by Using ISSR Marker
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Authors
Affiliations
1 MGM College of Agricultural Biotechnology, Aurangabad (M.S.), IN
2 College of Agriculture Biotechnology, Latur (M.S.), IN
3 Banana Research Station, Nanded (M.S.), IN
4 Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), IN
1 MGM College of Agricultural Biotechnology, Aurangabad (M.S.), IN
2 College of Agriculture Biotechnology, Latur (M.S.), IN
3 Banana Research Station, Nanded (M.S.), IN
4 Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), IN
Source
Asian Journal of Bio Science, Vol 5, No 1 (2010), Pagination: 138-139Abstract
The present study was carried out with emphasis on ISSR profiling and genetic relationship studies on pearl millet using ISSR marker. The experimental material consisted of 15 genotypes of pearl millet. The seeds of 15 genotypes of pearl millet were grown in petriplates and the young leaves from them were used for DNA extraction. Extraction of total genomic DNA was carried out following the methods suggested by Saghai-Maroof et al. (1984) with minor modification. The selected primers generated 123 ISSR bands and the size of the amplification products ranged from 150-35000 bp. The number of bands generated per primer ranged from 09 (CA)6RG to 19 (AC)8YA with a mean of 13.6 bands per primer. Out of the bands generated 80 were polymorphic and 43 were monomorphic. The genotype GHB 235 showed maximum similarity (0.92) whereas, minimum was for ICMV 155 (0.55) among the genotypes studied.Keywords
Pearl Millet, Genetic Diversity, ISSR.- In Vitro Studies on Callus Induction and Plantlet Regeneration in Safflower (Carthamus tinctorius L.)
Abstract Views :396 |
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Authors
Affiliations
1 Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), IN
2 Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), IN
3 Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), IN
1 Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), IN
2 Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), IN
3 Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), IN
Source
Asian Journal of Bio Science, Vol 3, No 1 (2008), Pagination: 231-232Abstract
Callus induction and regeneration in safflower (Carthamus tinctorius L.) cv. Sharda and PBN-12 was optimized using various explants such as hypocotyls, cotyledonary leaves ischolar_mains and apical shoot buds. The cotyledonary leaves exhibited better response for callus induction as compare to ischolar_mains and hypocotyls on MS supplemented with 5mgl-1 NAA, 0.25mg-1 BAP and 1% sucrose.Keywords
Callus Induction, Plantlet Regeneration, Safflower.- Cryopreservation of Citrus reticulata Embryonic Axes by Vitrification Technique
Abstract Views :218 |
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Authors
Affiliations
1 Department of Agril. Biotechnology, Marathwada, Agriculture University Parbhani - 431 402 (M.S.), IN
1 Department of Agril. Biotechnology, Marathwada, Agriculture University Parbhani - 431 402 (M.S.), IN