Asian Journal of Pharmaceutical Research and Health Care

Kalyana Ramu Buridi

Abstract

Two simple and sensitive visible spectrophotometric methods are described for the determination of tianeptine in bulk and pharmaceutical preparations based on the formation of colored species with SA- NaNO ₂ - α - NA (M ₁ ) or SNP-acetaldehyde reagent (M ₂ ) under specified experimental conditions and exhibiting λ _max at 479 nm or 555nm respectively. The Regression analysis of Beer's Law plot showed good correlation in a general concentration range of 10-30μg/ml or 8.0-24μg/ml with correlation coefficient (r ² = 0.999) for methods M ₁ and M ₂ respectively. The proposed methods are applied to commercial available stablon tablets and the results are statistically compared with those obtained by the UV reference method and validated with respect to accuracy, precision, linearity, limit of detection, percentage of recovery, repeatability and recovery studies. The results are found satisfactory and reproducible. These methods are applied successfully for the estimation of tianeptine in tablet form without the interference of excipients.

Keywords

Assay, Acetaldehyde, Coupling, Diazotization, Regression Equation, Sodium Nitroprussi.

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Kalyana Ramu Buridi

Abstract

In the present investigation, a direct, simple and sensitive visible spectrophotometric method without using any buffer solution is described for the assay of tenofovir disproxil fumarate in pure and tablet dosage forms. The method is based on the formation of yellowish brown colored species by the drug with NQS reagent and exhibits absorption maxima at 451 nm. Regression analysis of Beer-Lambert plots showed good correlation in the concentration ranges (8-40) μg/ml. The proposed method is applied to commercial available tablets and the results are statistically compared with those obtained by the reported UV reference method and validated by recovery studies. The results are found satisfactory and reproducible. The method is applied successfully for the estimation of the tenofovir disproxil fumarate in the presence of other ingredients that are usually present in dosage forms. The method offers the advantages of rapidity, simplicity and sensitivity and normal cost and can be easily applied to resource-poor settings without the need for expensive instrumentation and reagents.

Keywords

Assay, Beer's Law, Chemotherauptic Agent, NQS Reagent, Regression Equation, Statistical Analysis.

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