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Recently, accumulating evidences indicated that microRNA-141 (miR-141) is associated with NPC due to their abilities to affect the expression of genes that modulate tumorigenesis. Unfortunately, there is still limited publication about miR-141 expression in Vietnamese nasopharyngeal cancer patients. In this study, we adopted bioinformatics tools, such as Pictar, Target Scan, miRDB, etc. to predict its target gene. As the results, PTEN (phosphatase and tensin homolog gene), acts oncogenic role associated with biological processes lead to the nasopharyngeal carcinogenesis, was identified as the direct target of miR-141. Experimentally, we reported the evaluation of miR-141 and PTEN expression in NPC biopsy samples and non-cancerous swab samples. The present study demonstrated that miR-141 was upregulated 9.38 times, and PTEN expression was significantly lowered in NPC biopsy samples compared to non-cancerous epithelial swab samples. Our finding demonstrated that the miR-141 was upregulated and PTEN was down regulated in NPC biopsy samples. In the upcoming research, a larger clinical sample size from patients at each stage of the NPC will be performed to understand the expression pattern of the miR-141 and PTEN for further applied in early diagnosis and prognosis as well as therapeutic of NPC.

Keywords

miR-141, Nasopharyngeal Carcinoma, PTEN.

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First Record of Identification of RPMS1 Gene Variations in Vietnamese Nasopharyngeal Carcinoma Patients

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Authors

Duc Thuan Lao ¹, Danh Hoang Nguyen ¹, Minh Trong Quang ¹, Hue Hong Thieu ², Thuy Ai Huyen Le ¹

Affiliations
1 Ho Chi Minh City Open University, 97 Vo Van Tan Str., Ward 6, District 3 Ho Chi Minh City − 700000, VN
2 University of Science, Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu Str., Ward 4, District 5 − 700000, VN

Source

Asian Journal of Pharmaceutical Research and Health Care, Vol 10, No 4 (2018), Pagination: 97-103

Abstract

Epstein-Barr Virus (EBV) infection and EBV genes’ variation are considered as the etiological factors contributed to Naso- Pharyngeal Carcinoma (NPC). In the latent EBV stage, not only the latent membrane proteins but also RPMS1 expression has been confirmed in all EBV-associated tumors. In Vietnam, an Asian country with the high incidence, mortality rate of NPC, had limit research on the RMPS1 gene variation. Therefore, the objects of current study were to identify the pattern of RMPS1 variations in Vietnamese NPC patients for its value further applied in NPC patients. In this study, thirty NPC biopsy samples and thirty non-cancerous swab specimens were collected from local patients, analyzed by PCR, sequencing and compared to previous B95-8 sequence. As the results, the strongly association between the detection of RPSM1 gene and NPC incidence in Vietnamese NPC patients was determined. Additionally, the RMPS1 gene variants, including wild-type, RMPS1-B, RMPS1-C, RMPS1-C*, were identified. Among them, RMPS1-C/C* was preferential in Vietnamese nasopharyngeal cancer. In conclusion, those data is the first dataset on the polymorphism in the RPMS1 gene in Vietnamese NPC patients, and could be utilized as a promising biomarker for prognosis, diagnosis and therapy for NPC based on the EBV gene variations.

Keywords

Nasopharyngeal Carcinoma, RPMS1, Variations.

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