Asian Journal of Pharmaceutical Research and Health Care

K. Ravi Shankar , A. Lakshmana Rao , L. Kalyani

Abstract

The hepatoprotective activity of ethanolic extract of fruits of Alstonia scholaris was evaluated by carbon tetrachloride (CCL4) - induced hepatoto xicity in rats. The toxicant CCL4 was used to induce hepatotoxicity at a dose of 2 ml/kg as 1:1 mixture with olive oil. The ethanolic extract of fruits of Alstonia scholaris was administered in the dose of 150 and 300 mg/kg/day orally for 5 days. Silymarin (50 mg/kg) was used as standard drug. The hepatoprotective effect of th e ethanolic extract was evaluated by the assessment of biochemical parameters such as SGOT, SGPT, SALKP , total bilirubin and histopathological studies of the liver. Treatment of animals with the ethanolic extract significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver as indicated by lower levels of serum bilirubin as compared with the normal and silymari n treated groups.

Keywords

Carbon Tetrachloride, Hepatoprotective Activity, Alstonia scholaris, Silymarin.

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A. Lakshmana Rao , B. N. V. Ravi Kumar , G. Girija Sankar

Abstract

A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of gatifloxacin in its pharmaceutical dosage forms. In this method RP - C ₁₈ column (150mmx4.6mm I.D., 5 μm particle size) with mobile phase consisting of acetonitrile and 0.05M phosphate buffer in the ratio of 25:75 v/v in isocratic mode was used. The detection wavelength is 293nm and the flow rate is 0.8ml/min. Ciprofloxacin is used as internal standard. The linearity was fo und to be in the range of 0.1 to 10 μg/ml and shows a correlation coefficient of 0.9999. The intra - and inter - day variation was found to be less than 1.2% showing high precision of the assay method. The mean percent recovery of the drug from the solution c ontaining 4 μg/ml was 100.5 ± 2.06 indicating high accuracy of the proposed method. Hence, this method is simple, fast, specific, accurate, precise and less time consuming for the estimation of gatifloxacin in pharmaceutical dosage forms.

Keywords

HPLC, Gatifloxacin, Estimation, Pharmaceutical Dosage Forms.

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A. Lakshmana Rao , P. Vijay Srinivas , J. V. L. N. S. Rao

Abstract

A simple, rapid, sensitive and precise high performance liquid chromatographic (HPLC) method has been developed for the estimation of mycophenolate mofetil in bulk and tablet dosage form. In this method RP-C₁₈ column (250mmx4.6mm I.D., 5im particle size) with mobile phase consisting of acetonitrile and 0.03M phosphate buffer in the ratio of 60:40 v/v in isocratic mode was used. The detection wavelength is 254 nm and the flow rate is 0.8 ml/min. In the range of 20-120μg/ml, the linearity of mycophenolate mofetil shows a correlation coefficient of 0.9995. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters. The mobile phase selected for the method is simple, fast, accurate and precise and hence can be applied for routine quality control analysis of mycophenolate mofetil in bulk and its tablet dosage form.

Keywords

HPLC, Mycophenolate Mofetil, Validation.

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A. Lakshmana Rao , G. G. Sankar

Abstract

The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells. Human colonic adenocarcinoma cells that are able to express differentiation features characteristic of mature i ntestinal cells, such as enterocytes or mucus cells. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug tra nsport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.

Keywords

Caco-2 Cells, Passive Transport, Apoptosis.

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