Asian Journal of Pharmaceutical Research and Health Care

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Comparative Experimental Studies of Few L-Type and T-Type Ca2+ Channel Blockers against In-Ovo and In-Vitro Models of Angiogenesis


Affiliations
1 Department of Pharmacology and Toxicology, Northern Border University, Rafha, Saudi Arabia
2 Department of Pharmaceutical Chemistry, Northern Border University, Rafha, Saudi Arabia
 

Angiogenesis is the development of new blood vessels from pre-existing one. The ion-channels on endothelium plays vital role in cell proliferation and related angiogenesis. We aimed to investigate the effects of L-type (Verapamil and Diltiazem) and T-type (Ethosuximide) Calcium Channel Blockers (CCBs) on neovascularization. The effects on neovascularization were investigated by in-ovo (CAM, Chick Chorioallantoic membrane) and in-vitro (aortic ring assay) methods. Each test drug was tested for at least 3 doses and the anti-angiogenic effect was compared with Suramin as standard and normal control groups. Various vital parameters were recorded during the experiment like the number of blood capillaries, sprouts formation, angiogenic score etc. The L-type Ca2+ channel blockers Verapamil at the dose of 50μM, 110 and 220 μM/disk has shown significant (p < 0.001) reduction in the number of branching points in CAM assay. For the further confirmation, angiogenic activity was evaluated in vitro by rat aortic ring assay method; the area of sprouts was reduced by the medium and high dose of verapamil. Diltiazem has demonstrated modest anti-angiogenic activity by both the models, whereas T-type calcium channel blocker ethosuximide has not shown any effect on the neovascularization. Among all the tested drugs verapamil has shown the promising anti-angiogenic property. Thus verapamil and diltiazem may have antiangiogenic activity defines novel beneficial effects in angiogenic mediated pathological conditions in addition to their main indications in cardiovascular complications.

Keywords

Angiogenesis, CAM Assay, L-Type Ca2+ Channel Blockers, Rat Aortic Ring Assay, T-Type Calcium Channel Blocker.
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  • Comparative Experimental Studies of Few L-Type and T-Type Ca2+ Channel Blockers against In-Ovo and In-Vitro Models of Angiogenesis

Abstract Views: 261  |  PDF Views: 121

Authors

Vijay R. Chidrawar
Department of Pharmacology and Toxicology, Northern Border University, Rafha, Saudi Arabia
Mohd. Imran
Department of Pharmaceutical Chemistry, Northern Border University, Rafha, Saudi Arabia

Abstract


Angiogenesis is the development of new blood vessels from pre-existing one. The ion-channels on endothelium plays vital role in cell proliferation and related angiogenesis. We aimed to investigate the effects of L-type (Verapamil and Diltiazem) and T-type (Ethosuximide) Calcium Channel Blockers (CCBs) on neovascularization. The effects on neovascularization were investigated by in-ovo (CAM, Chick Chorioallantoic membrane) and in-vitro (aortic ring assay) methods. Each test drug was tested for at least 3 doses and the anti-angiogenic effect was compared with Suramin as standard and normal control groups. Various vital parameters were recorded during the experiment like the number of blood capillaries, sprouts formation, angiogenic score etc. The L-type Ca2+ channel blockers Verapamil at the dose of 50μM, 110 and 220 μM/disk has shown significant (p < 0.001) reduction in the number of branching points in CAM assay. For the further confirmation, angiogenic activity was evaluated in vitro by rat aortic ring assay method; the area of sprouts was reduced by the medium and high dose of verapamil. Diltiazem has demonstrated modest anti-angiogenic activity by both the models, whereas T-type calcium channel blocker ethosuximide has not shown any effect on the neovascularization. Among all the tested drugs verapamil has shown the promising anti-angiogenic property. Thus verapamil and diltiazem may have antiangiogenic activity defines novel beneficial effects in angiogenic mediated pathological conditions in addition to their main indications in cardiovascular complications.

Keywords


Angiogenesis, CAM Assay, L-Type Ca2+ Channel Blockers, Rat Aortic Ring Assay, T-Type Calcium Channel Blocker.

References





DOI: https://doi.org/10.18311/ajprhc%2F2018%2F19587