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Development and Validation of Simple HPLC Method for Estimation of Flubendiamide in Blood
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Flubendiamide, novel insecticide used for control of lepidopteron insects on agriculture crops in India. Flubendiamide exposure of to human and animals occurs through ingestion of contaminated feed/fodder, food-products and drinking water etc. Aim of the present study was to develop a simple HPLC method for flubendiamide estimation in blood plasma. Result indicated that HPLC estimation of flubendiamide in blood plasma shows linear standard curve in the range of 0.039-5.0 μg.ml-1 with correlation coefficient (R2) 0.999. Limit of Detection (LOD) and Limit of Quantification (LOQ) was 0.039 μgml-1. Intra-day, inter-day coefficient of variance and mean recovery were found to be 2.70%, 3.50% and approx. 100% respectively, by using C18 column and mixture of acetonitrile: water (70:30, v/v) as mobile phase with flow rate of 0.5 mlmin-1 at 210 λ. Result of this study concluded that this HPLC method is very simple, reliable and repeatable for flubendiamide estimation blood plasma.
Keywords
Flubendiamide, Plasma, HPLC
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