Toxicology International (Formerly Indian Journal of Toxicology)

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Allopurinol Reduced Oleic Acid Plasma on High-fat Diet-induced Hypertensive Rats


Affiliations
1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok – 16424, Indonesia
2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok – 1642, Indonesia
     

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Hypertension is still a big challenge and is a key risk factor for cardiovascular disease which is the leading cause of death in the world. We evaluate the effects of Allopurinol in high-fat diet-induced hypertensiverats. To this end we induced hypertension of SD rats by high fat-diet for six weeks and divided into 6 groups (n = 5), for six weeks and one group was fed standard chow. Drugs were given for seven days orally; one group was only given a high-fat diet and CMC 0.5%, as Negative Control; one group was given a high-fat diet and Isosorbide Dinitrate (ISDN) 18 mg/kg as Positive Control, three groups were given a high-fat diet and Allopurinol with dose range of 45-180 mg/kg. Systolic, diastolic, mean arterial pressure and blood glucose were compared between diet groups and normal group; Free Fatty Acid Plasma and Amino Acid Plasma were determined by GC-MS. As a results, Allopurinol significantly reduced blood pressure in all three dose groups (p<0.05). The dose group of Allopurinol and ISDN gave similar results in lowering blood pressure and glucose levels which measured on the last day of drug administration. Allupurinol also reduced oleic acid plasma compared with high-fat diet group. In conclusion, Allopurinol has a potential as an antihypertensive agent by modulating linoleic acid.

Keywords

Allopurinol, Blood Pressure, High-fat Diet, Hypertension, Linoleic Acid
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  • Whelton PK, Carey RM, Aronow WS, Ovbiagele B, Casey DE, Smith SC, et al. Guideline for the prevention, detection, evaluation, and management of high blood pressure in adults. Journal of American College of Cardiology. 2017. 283 p.

  • Lontchi-Yimagou E, Tanya A, Tchankou C, Ngondi J, Oben J. Metabolic effects of quail eggs in diabetes-induced rats: Comparison with chicken eggs. Food Nutr Res. 2016; 60(1). PMid: 27717410 PMCid: PMC5055609. https://doi.org/10.3402/fnr.v60.32530

  • Soleimani M. Dietary fructose, salt absorption and hypertension in metabolic syndrome : Towards a new paradigm. Acta Physiologica. 2011; 201(1):55–62. PMid: 21143427. https://doi.org/10.1111/j.1748-1716.2010.02167.x

  • Zhao JD, Dong PS, Jia JJ, Zhao D, Wang HL, Li DL, et al. Allopurinol to influence blood pressure in hypertension patients: Systematic review and meta-analysis. Int J Clin Exp Med. 2018; 11(3):1390–400.

  • Wallwork CJ, Parks DA, Schmid-Schonbein GW. Xanthine oxidase activity in the dexamethasone-induced hypertensive rat. Microvasc Res. 2003; 66(1):30–7. https://doi.org/10.1016/S0026-2862(03)00019-0

  • Mondanelli G, Iacono A, Carvalho A, Orabona C, Volpi C, Pallotta MT, et al. Amino acid metabolism as drug target in autoimmune diseases. Autoimmun Rev [Internet]. 2019; 18(4):334–48. PMid: 30797943. https://doi.org/10.1016/j.autrev.2019.02.004 http://www.sciencedirect.com/science/article/pii/S1568997219300308

  • Amin AM, Mostafa H, Arif NH, Abdul Kader MASK, Kah Hay Y. Metabolomics profiling and pathway analysis of human plasma and urine reveal further insights into the multifactorial nature of coronary artery disease. Clin Chim Acta [Internet]. 2019; 493:112–22. PMid: 30826371. http://www.sciencedirect.com/science/article/pii/S0009898119300920 https://doi.org/10.1016/j.cca.2019.02.030

  • Teymoori F, Asghari G, Mirmiran P, Azizi F. High dietary intake of aromatic amino acids increases risk of hypertension. J Am Soc Hypertens [Internet]. 2018; 12(1):25–33. PMid: 29208471. http://www.sciencedirect.com/science/article/pii/S1933171117304072 https://doi.org/10.1016/j.jash.2017.11.004

  • Huang WC, Chen YL, Liu HC, Wu SJ, Liou CJ. Ginkgolide C reduced oleic acid-induced lipid accumulation in HepG2 cells. Saudi Pharm J [Internet]. 2018; 26(8):1178–84. PMid: 30532639 PMCid: PMC6260475. https://doi.org/10.1016/j.jsps.2018.07.006 http://www.sciencedirect.com/science/article/pii/S1319016418301452

  • Kaufman LN, Peterson MM, Smith SM. Hypertension and sympathetic hyperactivity induced in rats by high-fat or glucose diets. Am J Physiol Metab. 2017; 260(1):E95–100. PMid: 1987797. https://doi.org/10.1152/ajpendo.1991.260.1.E95

  • Malkoff J. Non invasive blood pressure measurement for mice and rats. Ide A, Conroy MJ, eds. Torrington: Kent Scientific Corporation; 2008. p. 1–12.

  • El-Bassossy HM, Mahmoud MF, Eid BG. The vasodilatory effect of Allopurinol mediates its antihypertensive effect: Effects on calcium movement and cardiac hemodynamics. Biomed Pharmacother. 2018; 100(Jan):381–7. PMid: 29454286. https://doi.org/10.1016/j.biopha.2018.02.033

  • El-Bassossy HM, Shaltout HA. Allopurinol alleviates hypertension and proteinuria in high fructose, high salt and high fat induced model of metabolic syndrome. Transl Res. 2015; 165(5):621–30. PMid: 25528722. https://doi.org/10.1016/j.trsl.2014.11.008

  • Beattie CJ, Fulton RL, Higgins P, Padmanabhan S, Mccallum L, Walters MR, et al. Allopurinol Initiation and change in blood pressure in older adults with hypertension. 2014; 64(5):1102–7. PMid: 25135183. https://doi.org/10.1161/ HYPERTENSIONAHA.114.03953

  • Katsuyuki M, Jeremiah S, Hideaki N, Paul E, Hirotsugu U, Queenie C, et al. Relationship of dietary linoleic acid to blood pressure. Hypertension [Internet]. 2008 Aug; 52(2):408– 14. PMid: 18606902 PMCid: PMC6668335. https://doi.org/10.1161/HYPERTENSIONAHA.108.112383

  • Iacono JM, Dougherty RM. Effects of polyunsaturated fats on blood pressure. Annu Rev Nutr [Internet]. 1993 Jul; 13(1):243–60. PMid: 8369147. https://doi.org/10.1146/ annurev.nu.13.070193.001331

  • Tawa M, Furukawa T, Tongu H, Sugihara M, Taguwa S, Tanaka R, et al. Stimulation of nitric oxide-sensitive soluble guanylate cyclase in monocrotaline-induced pulmonary hypertensive rats. Life Sci. 2018; 203:203–9. PMid: 29705351. https://doi.org/10.1016/j.lfs.2018.04.045

  • Goharinia M, Zareei A, Rahimi M, Mirkhani H. Can Allopurinol improve retinopathy in diabetic rats? Oxidative stress or uric acid; which one is the culprit? 2017; 12(Jan):401–8. PMid: 28974978 PMCid: PMC5615870. https://doi.org/10.4103/1735-5362.213985

  • Siguel E. A new relationship between total/high density lipoprotein cholesterol and polyunsaturated fatty acids. Lipids [Internet]. 1996 Jan; 31(1Part1):S51–6. PMid: 8729094. https://doi.org/10.1007/BF02637051


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  • Allopurinol Reduced Oleic Acid Plasma on High-fat Diet-induced Hypertensive Rats

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Authors

Affiati Noviarini
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok – 16424, Indonesia
Tri Wahyuni
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok – 16424, Indonesia
Anton Bahtiar
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok – 1642, Indonesia

Abstract


Hypertension is still a big challenge and is a key risk factor for cardiovascular disease which is the leading cause of death in the world. We evaluate the effects of Allopurinol in high-fat diet-induced hypertensiverats. To this end we induced hypertension of SD rats by high fat-diet for six weeks and divided into 6 groups (n = 5), for six weeks and one group was fed standard chow. Drugs were given for seven days orally; one group was only given a high-fat diet and CMC 0.5%, as Negative Control; one group was given a high-fat diet and Isosorbide Dinitrate (ISDN) 18 mg/kg as Positive Control, three groups were given a high-fat diet and Allopurinol with dose range of 45-180 mg/kg. Systolic, diastolic, mean arterial pressure and blood glucose were compared between diet groups and normal group; Free Fatty Acid Plasma and Amino Acid Plasma were determined by GC-MS. As a results, Allopurinol significantly reduced blood pressure in all three dose groups (p<0.05). The dose group of Allopurinol and ISDN gave similar results in lowering blood pressure and glucose levels which measured on the last day of drug administration. Allupurinol also reduced oleic acid plasma compared with high-fat diet group. In conclusion, Allopurinol has a potential as an antihypertensive agent by modulating linoleic acid.

Keywords


Allopurinol, Blood Pressure, High-fat Diet, Hypertension, Linoleic Acid

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i1%262%2F24194