Toxicology International (Formerly Indian Journal of Toxicology)

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Screening of Antimicrobial and Synergistic Activity of Withania somnifera, Aloe vera Plant Extracts and Certain Lactic Acid Bacterial Strains against Food Borne Pathogens


Affiliations
1 Department of Veterinary Public Health, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
2 Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
3 Department of Veterinary Public Health, School of Public Health and Zoonosis, GADVASU, Hyderabad, Telangana, India
4 Livestock Products Technology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
     

   Subscribe/Renew Journal


The methanolic extract of plants Withania somnifera, Aloe vera and certain lactic acid bacterial (LAB) strains were evaluated for their antibacterial and synergistic effect against food borne pathogens. Antagonistic/synergistic activity of plant extracts and LAB were tested alone and combinely against selected food borne pathogens by using well diffusion method. The results exhibited that the extracts of Withania somnifera, Aloe vera and the lactic acid bacterial strainswere showed excellentantimicrobial activity when they are tested alone against the test organisms.But the results obtained from the combined testing of methanolic extracts of plants and Lactic acid bacterial strains significantly lesser antibacterial activityagainst all the tested organisms except with Pediococciacidolactici 252 which showed synergistic activity with methonalic extract of Withania somnifera against E.coli. The present study indicated that the methanolic extracts of Withania somnifera, Aloe vera and LAB showed good antibacterial activity when they are used individually then the combined and these can be used as a natural drug for the treatment of several infectious diseases.

Keywords

Antimicrobial Activity, Food Borne, Withanea somnifera, Aloe vera, LAB.
User
Subscription Login to verify subscription
Notifications
Font Size

  • Al-Allaf M. A. H., Al-Rawi A. M. M and Al-Mola, A. T. Antimicrobial activity of lactic acid bacteria isolated from minced beef meat against some pathogenic bacteria. Iraqi J. Vet. Sci., 2009.23:115-117.

  • `Chan, B. K., S. T. Abedon, and C. Loc-Carrillo. Phage cocktails and the future of phage

  • therapy. Future Microbiology 2013.8(6):769-783.

  • Cotter, P. D., R. P. Ross, and C. Hill. Bacteriocins—a viable alternative to antibiotics?Nature Reviews Microbiology. 2013.11:95-105.

  • Dwivedi S. D, Sheeraz Ah. Wagay. Antimicrobial activity of leaf extracts of Jurinea dolomiaea plant against clinical and phytopathogenic bacteria. Chemical and Process Engineering Research 2014.24:9-13.

  • Kadouri, D. E., K. To, R. M. Q. Shanks, and Y. Doi. Predatory bacteria: A potential ally

  • against multidrug-resistant gram-negative pathogens. PLoS One.2013.8(5).

  • Line JE, Svetoch EA, Eruslanov BV, Perelygin VV, Mitsevich EV, Mitsevich IP. Isolation and purification of enterocin E-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria. Antimicrob Agents chemother.2008. 52: 1094-1100.

  • Owais, M., Sharad, K.S., Shehbaz,A. &Saleemuddin, M.Antibacterial efficacy of Withania somnifera(aswagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 2005.12:229-235.

  • Rajendran NK, Ramakrishnan J. In vitro evaluation of antimicrobial activity of crude extracts of medicinal plants against multi drug resistant pathogens. BiyolojiBilimleriAraptyrma Dergisi, 2009.2: 97-101

  • Rudrangshu Chatterjee, Dushyant Singh, Amita Gaurav Dimri, AnkitaPandita, Shiwani

  • Chaudhary and M.L.Aggarwal. Comparative study of antimicrobial activity of aloe vera gel and antibiotics against isolates from fast food. World J. Pharmacy and pharmaceutical Sci.2015.4 (4): 1058-1073.

  • Saranya S. and Hemashenpagam N. Antagonistic activity and antibiotic sensitivity of Lactic acid bacteria from fermented dairy products. Advances in Appli.Sci.Research, 2011.2:528-534.

  • Sarkono Faturrahman Yayansofyan. Isolation and identification of lactic acid bacteria from abalone (Haliotisasinina) as a potential candidate of probiotic. J. Biosci., 2010.2:38-42.

  • Satish S, Raghavendra MP, Mohana DC and Raveesha KA. Antifungal activity of a known medicinal plant Mimusopselengi L. against grain moulds. J. Agri. Technol. 2008.4(1), 151-165.

  • Schneitz, C. Competitive exclusion in poultry–– 30 years of research. Food Control 2005.16(8):657- 667.

  • Shariff, Z.U. Modern Herbal Therapy for Common Ailments. Nature Pharmacy Series, Spectrum Books Limited, Ibadan, Nigeria in Association with Safari Books (Export) Limited, United Kingdom.2001.1: 9-4.

  • Sinha S.N. (2012). Screening of antioxidant and antibacterial activities of various extracts of Withania somnifera. International journal of pharmacology and therapeutics, 2(1):36-42.

  • Sukhvir Kaur, Harjot Pal Kaur and Surbhi Aggarwal 2015. Evaluation of antibacterial activity, antioxidant potential and phytochemicals of withania somnifera. World J. pharmacy and pharmaceutical Sci.2012.4(3):1032-1042.

  • Thirupathi S, Ramasubramanian V; Sivakumar T; Thirumalaiarasu V. J. Biosci. Res. 2010, 1(4):251-258.


Abstract Views: 285

PDF Views: 1




  • Screening of Antimicrobial and Synergistic Activity of Withania somnifera, Aloe vera Plant Extracts and Certain Lactic Acid Bacterial Strains against Food Borne Pathogens

Abstract Views: 285  |  PDF Views: 1

Authors

B. Srinu
Department of Veterinary Public Health, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
K. Kasturi Devi
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
J. S. Bedi
Department of Veterinary Public Health, School of Public Health and Zoonosis, GADVASU, Hyderabad, Telangana, India
T. Madhava Rao
Department of Veterinary Public Health, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India
K. Kondal Reddy
Livestock Products Technology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India

Abstract


The methanolic extract of plants Withania somnifera, Aloe vera and certain lactic acid bacterial (LAB) strains were evaluated for their antibacterial and synergistic effect against food borne pathogens. Antagonistic/synergistic activity of plant extracts and LAB were tested alone and combinely against selected food borne pathogens by using well diffusion method. The results exhibited that the extracts of Withania somnifera, Aloe vera and the lactic acid bacterial strainswere showed excellentantimicrobial activity when they are tested alone against the test organisms.But the results obtained from the combined testing of methanolic extracts of plants and Lactic acid bacterial strains significantly lesser antibacterial activityagainst all the tested organisms except with Pediococciacidolactici 252 which showed synergistic activity with methonalic extract of Withania somnifera against E.coli. The present study indicated that the methanolic extracts of Withania somnifera, Aloe vera and LAB showed good antibacterial activity when they are used individually then the combined and these can be used as a natural drug for the treatment of several infectious diseases.

Keywords


Antimicrobial Activity, Food Borne, Withanea somnifera, Aloe vera, LAB.

References





DOI: https://doi.org/10.22506/ti%2F2017%2Fv24%2Fi3%2F166507