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Zeino, Souad
- Spectrophotometric Determination of Glimepiride in Pure and Pharmaceutical Formulations using Bromophenol Blue and Thymol Blue
Authors
1 Department of Chemistry, Faculty of Sciences, University of Aleppo, SY
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1169-1177Abstract
Two simple, direct and accurate spectrophotometric methods have been developed for the determination of Glimepiride (GLM) in pure and pharmaceutical formulations by ion-pair complexes with Bromophenol Blue (BPB) and Thymol Blue (TB). The methods involve the formation of yellow ion-pair complexes between BPB and TB with GLM at pH<3.8; after reacting GLM with Na2CO3 to give C24H33N4H+O5NaS which is prepared in chloroform. The formed complexes [GLM]:[BPB] in chloroform and [GLM]:[TB]2 in acetonitrile were measured at λmax 424 and 392 nm, respectively. Molar absorptivity(ε) for complexes were 23000 and 28000 L.mol-1.cm-1, respectively. Beer’s law was obeyed in the concentration range of 1.126 – 46.608 μg.mL-1 with relative standard deviations (RSD) ≤ 3.8% in presence of 5.0x10-4 mol.L-1 of BPB and 0.319 – 34.343 μg.mL-1 with RSD ≤ 4.0% in presence of 2.5x10-4 mol.L-1 of TB. The proposed method was validated for specificity, linearity, precision, accuracy, repeatability, sensitivity (LOD and LOQ) and robustness.
The results for the determination of GLM using TB were more sensitive and accurate than using BPB. The developed method is applicable for the determination of GLM in pure and different dosage forms with average assay of 99.0 to 101.0% and the results are in good agreement with those obtained by the RP-HPLC reference method.
Keywords
Direct Spectrophotometric Method Ion-Pair Complexes, Glimepiride, Bromophenol Blue, Thymol Blue.
References
- Meyer D, Thierry, 2014- Substituent effects on absorption spectra of pH indicators: An experimental and computational study of sulfonphthaleins dyes, Dyes Pigments, 102:241–250.
- Rahman N, Najmul S, Azmi H. Extractive spectrophotometric methods for determination of diltiazem HCl in pharmaceutical formulations using bromothymol blue, bromophenol blue and bromocresol green. J. Pharm. Biomed. Anal. 2000; 24: 33-41.
- Ashour S, Shehna MF, Bayram R. Spectrophotometric determination of alfuzosin HCl in pharmaceutical formulations with some sulphonephthalein dyes. Int. J. Biomed. Sci. 2006; 2: 273-278.
- Almasri IM, Al-Laham MK. Development and validation of spectrophotometric method for determination of cefixime and glimepiride by ternary complex formation with eosin and Cu(II). IAJPR. 2014; 12: 5670-5677.
- Ravindra N, Singhvi I. Spectrophotometric Estimation of Glimepiride from Pharmaceutical Dosage Forms. Asian J. Chem. 2008; 6: 4379-4382.
- Rahman N, Azmi SNH. Extractive spectrophotometric methods for determination of diltiazem HCl in pharmaceutical formulations using bromothymol blue, bromophenol blue and bromocresol green. J. Pharm. Biomed. Anal. 2000; 24: 33-41.
- Rahman N, Khan NA, Azmi SNH. Extractive spectrophotometric methods for the determination of nifedipine in pharmaceutical formulations using bromocresol green, bromophenol blue, bromothymol blue and eriochrome black T. Il Farmaco. 2004; 59: 47-54.
- Altinöz S, Tekeli D. Analysis of glimepiride by using derivative UV spectrophotometric method. J. pharm. Biomed. Anal. 2001; 24: 507-522.
- Sevgi TU. Spectrophotometric Determination of Glimepiride in Pharmaceutical Preparations Based on the Formation of Charge Transfer and Ion_Pair Complexes. J. Analyt. Chem. 2013; 68: 606-610.
- Bonfilio de Rudy B, Araujo Magali, Salgado Hérida RN. Development and Validation of an UV-Derivative Spectrophotometric Method for Determination of Glimepiride in Tablets. J. Braz. Chem. Soc. 2011; 22: 292-301.
- Siddiqui MR, AlOthman ZA, Rahman N. Analytical techniques in pharmaceutical analysis: A review, Arabian J. Chem. 2013; 10: 1409-1421.
- AL Othman ZA, Rahman N, Siddiqui MR. Review on pharmaceutical impurities, stability studies and degradation products: an analytical approach, Rev. Adv. Sci. Eng. 2013; 2: 155-221.
- Rahman N, Azmi SNH, Wu HF. The importance of impurity analysis in pharmaceutical products: an integrated approach. Accred. Qual. Assur. 2006; 11: 69-74.
- Ramadan AA, Zeino S. Direct Spectrophotometric Determination of Glimepiride in Pure Form and Pharmaceutical Formulations Using Bromocresol Purple. J. Adv. Chem. 2018; 15: 6186-6198.
- Ashour S, Chehna MF, Bayram R. Spectrophotometric Determination of Alfuzosin HCl in Pharmaceutical Formulations with some Sulphonephthalein Dyes. Int. J. Biomed Sci. 2006; 2: 273-281.
- Karthik A, Subramanian G, Mallikarjuna Rao C, Krishnamurthy Bhat, Ranjithkumar A, Musmade P, Surulivelrajan M, Karthikeyan K, Udupa N. Simultaneous determination of pioglitazone and glimepiride in bulk drug and pharmaceutical dosage form by RP-HPLC method. Pak J Pharm Sci. 2008; 21(4): 421-426.
- ICH: Proceedings of the International Conference on Harmonization of Technical Requirement of Registration of Pharmaceuticals for Human Use (ICH Harmonized Tripartite Guidelines) 2000.