Research Journal of Pharmacy and Technology

K. V. Bhaskara Rao ¹, S. Veena ², S. Pooja ², Y. Shriya ², R. Shivram ²

Affiliations
1 Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, TN - 632 014, IN
2 Molecular and Microbiology Research Laboratory, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, IN

Abstract

Research for the selection of different enzyme producing microorganisms are increased around the world. Marine actinomycetes are one of the most investigated group since they are considered as a potential source of biotechnologically interesting products. The aim of the present study was to isolate commercially useful enzymes producing marine actinobacteria from Kanyakumari marine sediments. A total of 10 actinobacterial colonies were isolated which were designated as PV-1 to PV-10. In primary screening PV1, PV5, PV7 and PV8 showed good activity for cellulase and lipase enzyme production with a zone of hydrolysis of 10 mm and above. The isolate PV7 showed maximum activity with a zone of hydrolysis of 20 mm and 19 mm for cellulase and lipase enzyme respectively. Among all isolates PV7 and PV10 showed higher protease enzyme production with a zone of hydrolysis 19 mm and 14 mm respectively. In secondary screening PV7 showed maximum activity of 18.65 U/ml for cellulase followed by 15.32 U/ml of protease and 11.34 U/ml of lipase activities. The potential isolate PV7 was identified as Actinomycetales bacterium-PV7 using molecular methods by 16S rRNA sequencing. This study concludes that marine actinobacterial can be considered as source for the production of industrial and pharmaceutical enzymes.

Keywords

Marine Actinobacteria, Lipase, Protease, Cellulase, Actinomycetales bacterium.

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N. Akshatha ¹, T. Niki ¹, S. Sripradha ¹, S. Veena ¹, K. V. Bhaskara Rao ¹

Affiliations
1 Molecular, Microbiology Research Laboratory, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, IN

Abstract

The present study focused on the isolation and extraction of biosurfactant producing bacteria. Bacterial colonies were isolated from oil-soaked soil sample collected from an automobile work shop in Bangalore, India on Nutrient Agar medium. A total of 8 bacterial colonies were isolated and were designated as ANS-1 to ANS-8. All isolates were then screened for biosurfactant production. The primary screening consisted of haemolytic assay, where isolates ANS1 and ANS6 showed β-haemolysis and isolates ANS2 and ANS5 showed α-haemolysis. For secondary screening, oil displacement test and drop collapse test and emulsification index calculation were conducted. Isolate ANS6 showed positive for drop collapse test and oil displacement test. ANS6 demonstrated highest emulsification index of 63.333%. Extracted biosurfactant was characterized using FTIR which showed glycerine as a cyclic lipopeptides. The potential isolate with high biosurfactant producing ability was identified using 16s rRNA sequencing as Bacillus amyloliquefaciens VITANS6.

Keywords

Biosurfactants, Haemolysis, Oil Contamination, Oil Displacement, Drop Collapse, Bacillus Amyloliquefaciens.

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