Refine your search
Collections
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sangeetha, S.
- Isolation and Screening the Pharmacological Activities of Vegetative and Spore-Crystal Proteins from Bacillus thuringiensis
Abstract Views :212 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, IN
1 Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 38-40Abstract
Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and spore-crystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.Keywords
Bacillus thuringiensis (Bt), Spore-Crystal Proteins, Vegetative Proteins. SDS-PAGE, Antioxidant.References
- Akiba T, Abe Y, Ktada S, Kusaka Y, Ito A, Ichimatsu T, Katayaman H, Akao T, Higuchi K, Mizuki E, Ohba M, Kanai R and Harata K. Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells. Journal of Molecular Biology. 2009; 386 (1): 121-33.
- Aronson AI and Shai Y. Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action. FEMS Microbiology Letters. 2001; 195: 1-8.
- Baum JA, Johnson TB and Carlton BC. Bacillus thuringiensis: Natural and recombinant bioinsecticide products. In: Biopesticides: Use and Delivery, Hall FR and Menn JJ, Eds, Humana Press 1999, Totowa, NJ, 189-210.
- Becker N. Bacterial control of vector mosquitoes and black flies In: Entomopathogenic Bacteria: From Laboratory to Field Application. Charles JF, Delecluse A, Neilson-LeRoux (Eds). Dordrecht, Kluwer Academic Publishers 2000; 383-98.
- Capello M, Bungiro RD, Harrison LM, Bischof LT, Griffitts JS, Barrows BD and Aroian RV. A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostomaceylanicus. Proc Natl AcadSci USA 2006; 103: 15154-9.
- Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus D, Baum J and Dean DH. Revision of the literature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiology Molecular Biology Review 1998; 62: 807-13.
- Jung YC, Mizuki E, Akao T and Cote JC. Isolation and characterization of a novel Bacillus thuringiensis strain expressing a novel crystal protein with cytocidal activity against human cancer cells. Journal of Applied Microbiology. 2007; 103: 65-79.
- Katayama H, Kusaka Y, Yokota H, Akao T, Kojima M, Nakamura O, Mekada E and Mizuki E. Parasporin-1, a novel cytotoxic protein from Bacillus thuringiensis, induces Ca2+ influx and a sustained elevation of the cytoplasmic Ca2+ concentration in toxin-sensitive cells. Journal of Biological Chemistry. 2007; 282: 7742-52.
- Kitada S, Abe Y, Shimada H, Kusaka Y, Matsuo Y, Katayama H, Okumura S, Akao T, Mizuki E, Kuge O, Sasaguri Y, Ohba M and Ito A. Cytocidal actions of parasporin2, an anti-tumour crystal toxin from Bacillus thuringiensis. Journal of Biological Chemistry 2006; 281(36): 26350-60.
- Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H and Park YS. Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cells. Journal of Applied Microbiology. 1999; 86: 477-86.
- Mizuki E, Park YS, Saitoh H, Yamashita S, Akao T, Higuchi K and Ohba M. Parasporin, a human leukaemic cell-recognizing parasporal protein of Bacillus thuringiensis. Clinical and Diagnostic Laboratory Immunology. 2000; 7: 625-34.
- Chondromalacia Patellae:A Review
Abstract Views :206 |
PDF Views:0
Authors
Affiliations
1 School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamilnadu, IN
2 Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, IN
1 School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-117, Tamilnadu, IN
2 Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 412-418Abstract
Chondromalacia patella (knee pain) is the softening and breakdown of the tissue (cartilage) on the underside of the kneecap (patella) and is often referred to as chondromalacia of the patella, patellofemoral syndrome, or runner's knee. Pain Results when the knee and the thigh bone (femur) rub together. Abnormal knee cap positioning, tightness or weakness of the muscles associated with the knee, too much activity involving the knee, and flat feet may increase the likelihood of chondromalacia patella. The undersurface of the patella is covered with hyaline cartilage that articulates with the hyaline cartilage covered femoral groove (trochlear groove). Post-traumatic injuries, microtrauma wear and tear, and iatrogenic injections of medication can lead to the development of chondromalacia. Chondromalacia occurs in any joint and is especially common in joints that have had trauma and deformities. Cartilage is the soft tissue padding which is present between all joint and bones and acts like a shock absorber. The cartilage experiences a lot of wear, tear and damage over time. The cartilage is essentially avascular (has no blood or nerve supply) and is therefore quite a difficult area to heal. Long term therapy is essential in ensuring healthy repair so that further complications are not experienced in the future.Keywords
Chondromalacia, Chondromalacia Patella, Knee Pain, Cartilage.References
- Duran S, Cavusoglu M, Kocadal O, Sakman B. Association between trochlear morphology and chondromalacia patella: an MRI study. Clin Imaging. 2017;41:7-10.
- Brody LT and Thein JM. Nonoperative treatment for patellofemoral pain. J Orthop Sports Phys Ther. 1998; 28: 336–344.
- Wittstein JR, O’Brien SD, Vinson EN, Garrett WE Jr. MRI evaluation of anterior knee pain: predicting response to nonoperative treatment. Skeletal Radiol. 2009; 38: 895–901.
- Centeno CJ, Busse D, Kisiday J, Keohan C, Freeman M, et al. Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells. Pain Physician. 2008; 11: 343–353.
- Pak J. Autologous adipose tissue-derived stem cells induce persistent bone- like tissue in osteonecrotic femoral heads. Pain Physician. 2012; 15:75–85.
- Fisher TF, Waterman BR, Orr JD, Holland CA, Bader J, Belmont PJ. Tibial Tubercle Osteotomy for Patellar Chondral Pathology in an Active United States Military Population. Arthroscopy. 2016; 32(11): 2342-2349.
- Wang Y, Sun JY, Zha GC. Results of Lateral Retinacular Release PlusCircumpatellarElectrocautery in Total Knee Arthroplasty without Patellar Resurfacing. J Knee Surg. 2017; 30(3):212-217.
- Reider B. A Pain in the Knee. Am J Sports Med. 2016; 44(5):1103-5.
- Petersen W, Ellermann A, Rembitzki IV, Scheffler S, Herbort M, Brüggemann GP, Best R, Zantop T, Liebau C. Evaluating the potential synergistic benefit of a realignment brace on patients receiving exercise therapy for patellofemoral pain syndrome: a randomized clinical trial. Arch Orthop Trauma Surg. 2016; 136(7):975-82.
- Crossley KM, Callaghan MJ, Van Linschoten R. Patellofemoral pain. Br J Sports Med. 2016; 50(4): 247-50.
- Glaviano NR, Kew M, Hart JM, Saliba S. Demographic and Epidemiological Trends In Patellofemoral Pain. Int J Sports PhysTher. 2015; 10(3):281-90.
- Antholz CR, Cherian JJ, Elmallah RK, Jauregui JJ, Pierce TP, Mont MA. Selective Patellar Resurfacing: A Literature Review. SurgTechnol Int. 2015; 26:355-60.
- Korean Food and Drug Administration (KFDA). Cell therapy: Rules and Regulations, Chapter 2, Section 14. Seoul, Korea, 2009; 4.
- Walsh CJ, Goodman D, Caplan AI, Goldberg VM. Meniscus regeneration in a rabbit partial meniscectomy model. Tissue Eng. 1999; 5: 327–337.
- Hong E, Kraft MC. Evaluating anterior knee pain. Med. Clin. North Am. 2014; 98(4):697-717.