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Nancy Immaculate Mary, R.
- Inhibition of Antibiofilm Mediated Virulence Factors in Pseudomonas aeruginosa by Andrographis Paniculata
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology & Avanced Studies. Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 1 (2017), Pagination: 141-144Abstract
The focal intent of the study is about Quorum sensing (QS) which is well known for biofilm production, bacterial virulence and antibiotic resistance. Inhibition of quorum sensing in bacteria can reduce the risk of pathogenicity in all bacterial infection. In the present study the methanolic leaf extract of Andrographis paniculata has showed its anti quorum sensing activity against acyl homoserine lactone -dependent showed a concentration dependent (10-100 μg/ml) reduction in biofilm production in Pseudomonas aeruginosa and the inhibition level was upto 10-80%. Microtiter plate (MTP)assay and Light microscopic analysis has further confirmed the antibiofilm activity in Pseudomonas aeruginosa when treated with methanolic leaf extract of Andrographis paniculata. In addition the methanolic ectract of Andrographis paniculata itself showed no antibacterial activity in Pseudomonas aeruginosa.Keywords
Antibiofilm, Quorum Sensing Inhibition, Acyl Homoserine Lactone, Pseudomonas aeruginosa, Microtiter Plate, Andrographis paniculata.
- Antiquorum Sensing Potential of Andrographolide from Andrographis paniculata in Vibrio harveyi
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 449-452Abstract
The recently discovered cell-cell communication system through signaling molecules among bacterial pathogens is known as Quorum sensing. It is well known for bacterial virulence, antibiotic resistance and biofilm production. Microbial pathogenicity is reduced by inhibiting the quorum sensing activity in all infections caused by bacteria. In this present study the compound Andrographolide which is present as a secondary metabolite in Andrographis paniculata was assessed for its anti quorum sensing activity against acyl homoserine lactone (AHL)-dependent biofilm formation and bioluminescence production in Vibrio harveyi (MTCC 7771). The Andrographolide showed a concentration dependent (100 μg/ml) reduction in biofilm production to a level of 97% and reduction of bioluminescence at the level of 98% in Vibrio harveyi (MTCC 7771) and compared with the standard Andrographolide (sigma aldrich). Light microscopic analysis and Microtiter plate assay further confirmed the reduction in the biofilm forming ability of Vibrio harveyi (MTCC 7771) when treated with andrographolide. The andrographolide at its standard concentration showed antibacterial effect on Vibrio harveyi(MTCC 7771).Keywords
Andrographolide, Andrographis paniculata, Vibrio harveyi, Bioluminescence, Microtiter Plate Assay.- Inhibition of Biofilm formation in Serratia marcescens by Andrographolide from Andrographis paniculata
Authors
1 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
2 Department of Biotechnology, Vels Institute of Science, Technology and Advanced Studies, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 789-791Abstract
Quorum sensing (QS) is well known for bacterial virulence, antibiotic resistance and biofilm production. Inhibition of quorum sensing can reduce the risk of microbial pathogenicity in all infection. In this present study the compound Andrographolide which is present in Andrographis paniculata leaf extract was assessed for its anti quorum sensing activity against acyl homoserine lactone (AHL)-dependent biofilm formation in Serratia marcescens. The Andrographolide obtained from Andrographis paniculata exhibited a concentration dependent (100 μg/ml) reduction in biofilm production in Serratia marcescens to a level of 90% and compared with the standard Andrographolide (sigma aldrich). Microtiter plate and Light microscopic analysis further confirmed the reduction in the biofilm forming ability of Serratia marcescens when treated with andrographolide. In addition the Andrographolide present in Andrographis paniculata by itself showed no growth inhibitory effect on Serratia marcescens.