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Ojha, Nupur
- Optimization and Characterization of Polyhydroxyalkanoates and its Copolymers Synthesized by Isolated Yeasts
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Authors
Nupur Ojha
1,
Nilanjana Das
1
Affiliations
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 3 (2017), Pagination: 861-868Abstract
The present study investigates the role of yeasts, isolated from different environments for its abilities to synthesize biodegradable polymer, Polyhydroxyalkanoates (PHA) and its copolymers. Serial dilution method and spread plate technique had been used for the isolation of yeasts from different samples. Screening had been done by crotonic assay followed by UV Spectrophotometric analysis. Growth parameters such as effect of carbon sources, incubation period, inoculum percentage, incubation temperature and pH of the production medium were optimized for each of the screened isolates. Characterization of PHA was done by FTIR and GCMS analysis. Maximum PHA produced was observed after 96 hours of incubation period inoculated with 2% (v/v) of inoculum in minimal media of pH 8 containing various carbon sources viz. 5% sucrose for Isolate1; 5% glucose for Isolate 2; 3% starch for Isolate 3 and 5% Lactose for Isolate 4. Incubation temperature was maintained at 37°C. Maximum PHA production was noted as 40 % in Isolate 1, 33% in Isolate 2, 29% in Isolate 3and 25% in Isolate 4 respectively on the basis of dry cell weight under optimized condition. Prominent peaks obtained through FTIR analysis at wavelength 3273, 2922, 1739 and 1462 cm -1 showed the presence of stretching bands of -OH-,-CH-, -C=O- and -CH3- group which are the characteristic peaks of PHA polymer. GC-MS chromatogram detected the peaks at retention time 3.063 and 19.25 min were corresponding to the derivatized products of butanoic acid (polyhydroxybutyrate) and octadecanoic acid (polyhydroxyoctadecanoate).The present study demonstrated that yeast Isolate 1 could produce high amount of PHA (40% on dry cell weight basis ) with hydroxybutyrate (HB) and hydroxyoctadecanoate (HOD) as its main constituents.Keywords
Yeast Isolates, Optimization, FTIR, GC-MS, Polyhydroxybutyrate, Polyhydroxyoctadecanoate.
- Green Synthesis of Antibacterial Silver Nanoparticles using Yeast Isolates and its Characterization
Abstract Views :198 |
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Bio-Medical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 83-92Abstract
The present study had investigated the role of yeasts, isolated from soil and food samples for its ability to synthesize silver nanoparticles. Serial dilution method followed by spread plate technique was used for isolation of yeasts on YEPD agar medium and the morphological characterization was done by simple staining technique. Primary screening was done by visual colour change followed by UV spectrophotometric analysis. Significant color change and prominent peaks around 420-450 nm were noted through UV spectrophotometric analysis in the cell free extract of Isolate 5, Isolate 8 and Isolate 13, revealed the synthesis of Ag-NPs. Optimization of growth parameters for the enhancement of Ag-NPs production was evaluated for each of the potent yeast isolates. Isolate 5 and 13 had showed highest Ag-NPs production after 24 h of incubation period, supplemented by fructose in the production medium of pH 9 along with 5mM substrate concentration of AgNO3, at 45˚C, whereas in case of isolate 8, highest production was observed with 3mM concentration of Ag NO3 at pH 8. FTIR analysis revealed the presence of significant peaks around 3441-3255, 2922-2964, 1576-1579, 1384-1415, 1276, 1153 and 1076cm-1 of Ag-NPs synthesized by isolate 5,8 and 13 respectively, were corresponding to the functional groups produced during the synthesis of Ag-NPs. XRD results showed peaks at 2 theta values 27.29°, 31.76°, 45.91°, 54.26-56.77° and 75.56-75.99°, were corresponding to (111), (200), (222), (311), and (331) lattice of silver nanoparticles. Distinct surface topology of the AgNPs synthesised by the potent yeast isolates were observed by AFM analysis. Positive anti-oxidant and anti-bacterial activity were elucidated by AgNPs synthesised by the potent yeast isolates, wherein AgNPs were found to be bacteriostatic at low concentration (5ug/ml) and bactericidal at high concentrations (100ug/ml).Keywords
Yeast Strains, Silver Nanoparticles, Optimization, FTIR, XRD, AFM, Antibacterial Activity.References
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- A Green Approach Towards Utilization of Floral Wastes for the Extraction of Natural Colorants
Abstract Views :172 |
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Authors
Affiliations
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
1 Bioremediation Laboratory, Department of Biomedical Sciences, School of Bio-Science and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 269-279Abstract
The present study is focused on the utilization of floral wastes for the extraction of natural colorants. Waste flowers were collected from various sites of temples, garden, domestic and decorative areas and identified as Rosahybrid, Tageteserecta, Spathodeacampanulata, Bougainvilleaglabra, and Euphorbiamili. Identification and characterization of extracted colorants was done using thin layer chromatography (TLC), UV Spectroscopy andFourier Transform Infra-red spectroscopy (FT-IR) analysis. Antimicrobial and antioxidant properties of extracted natural colorants were evaluated. Applicability of extracted colorants as natural dye and biofertilizer was also evaluated. It can be concluded that the natural colorants extracted from the floral wastes can be used in textile and pharmaceutical industries.In addition, contamination free natural colorants can also serve as safe biofertilizer.Keywords
Floral Wastes, Natural Colorants, Antimicrobial, Antioxidant, Natural Dye, Biofertilizer.References
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